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Predicting Skeletal Muscle and Whole-Body Insulin Sensitivity Using NMR-Metabolomic Profiling
Purpose: Abnormal lipoprotein and amino acid profiles are associated with insulin resistance and may help to identify this condition. The aim of this study was to create models estimating skeletal muscle and whole-body ...
Histone deacetylases 1 and 2 restrain CD4(+) cytotoxic T lymphocyte differentiation
Some effector CD4(+) T cell subsets display cytotoxic activity, thus breaking the functional dichotomy of CD4(+) helper and CD8(+) cytotoxic T lymphocytes. However, molecular mechanisms regulating CD4(+) cytotoxic T ...
Early suppression of immune response pathways characterizes children with prediabetes in genome-wide gene expression profiling
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Type 1 diabetes (T1D) is caused by autoimmune destruction of insulin-producing pancreatic p cells in the islets of Langerhans. Although defects in various T cell subsets have been linked to the disease pathogenesis, ...
A practical comparison of methods for detecting transcription factor binding sites in ChIP-seq experiments
Background: Chromatin immunoprecipitation coupled with massively parallel sequencing (ChIPseq)<br />is increasingly being applied to study transcriptional regulation on a genome-wide scale. While<br />numerous algorithms ...
A comprehensive evaluation of popular proteomics software workflows for label-free proteome quantification and imputation
<p>Label-free mass spectrometry (MS) has developed into an important tool applied in various fields of biological and life sciences. Several software exist to process the raw MS data into quantified protein abundances, ...
Critical evaluation of the subcutaneous engraftments of hormone naïve primary prostate cancer
<p><strong>Background:</strong> Patient-derived xenografts (PDXs) are considered to better recapitulate the histopathological and molecular heterogeneity of human cancer than other preclinical models. Despite technological ...
An unbiased in vitro screen for activating epidermal growth factor receptor mutations
Cancer tissues harbor thousands of mutations, and a given oncogene may
be mutated at hundreds of sites. Yet, only a few of these mutations have
been functionally tested. Here, we describe an unbiased platform for
the ...
Selective loss of kisspeptin signaling in oocytes causes progressive premature ovulatory failure
<p><strong>Study question: </strong>Does direct kisspeptin signaling in the oocyte have a role in the control of follicular dynamics and ovulation?</p><p><strong>Summary answer: </strong>Kisspeptin signaling in the oocyte ...
Quantitative Proteomics Reveals the Dynamic Protein Landscape during Initiation of Human Th17 Cell Polarization
Th17 cells contribute to the pathogenesis of inflammatory and autoimmune diseases and cancer. To reveal the Th17 cell-specific proteomic signature regulating Th17 cell differentiation and function in humans, we used a ...
Quantitative proteomics analysis of the nuclear fraction of human CD4+ cells in the early phases of IL-4-induced Th2 differentiation
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We used stable isotope labeling with 4-plex iTRAQ (isobaric tags for relative and absolute quantification) reagents and LC-MS/MS to investigate proteomic changes in the nucleus of activated human CD4(+) cells during ...