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Part 1: The role of amyloid fibrils in protein aggregation diseases and molecular therapy. Part 2: Isolation of single-chain Fraction variable antibodies that bind to the toxic 8 kDa-fragment of gelsolin using phage display
(2019-09-06)
Gelsolin amyloidosis (Meretoja’s disease) is an inherited disease with slowly developing neuromuscular symptoms. Patient’s carry a mutation leading to misfolding of the gelsolin protein, which initiates an aberrant proteolytic ...
DNA-avusteinen homogeeninen immunomääritys sydänperäiselle troponiini I:lle kelaattikomplementaatiota hyödyntäen
(Turun yliopisto, 2018-06-06)
Homogeenisissa immunomäärityksissä signaali muodostuu immunokemiallisten sitoutumisreaktioiden seurauksena, jolloin sitoutuneiden ja sitoutumattomien leimamolekyylien erottelu on tarpeetonta, ja määritykset ovat näin ollen ...
Bioaffiniteettimääritysten kiintokantajat ja geneeriset sitojat : Streptavidiinilla pinnoitetun mikrotiitterilevyn sitomisominaisuuksien parantaminen ja laadunvalvontamäärityksen kehittäminen
(Turun yliopisto, 2018-06-06)
Streptavidiini on Streptomyces avidinii-lajin erittämä tetrameerinen proteiini, jota käytetään geneerisenä sitojana in vitro-diagnostisissa kiintokantaja-määrityksissä. Sen sidos biotiiniä (vitamiini H) kohtaan on ...
Newborn screening for spinal muscular atrophy with EnLiteTM time resolved fluorescence resonance energy transfer polymerase chain reaction
(2019-03-13)
Spinal muscular atrophy (SMA) is a recessive neurodegenerative disorder, caused by mutations or a deletion in survival motor neuron (SMN) 1, the central gene for production of SMN protein. 95 % of all SMN cases are caused ...
Expanded genetic code and Effect of synonymous mutations on expression of Fab fragments
(2019-12-13)
Antigen binding fragment (Fab) is the part of an antibody which contains
an antigen binding site. Fab has many advantages over a conventional
antibody such as the reduced size, lack of glycosylation and therefore, ...
Unveiling the secrets of DNA : Improved expression and phage display efficiency of synthetic recombinant binding proteins in E. coli through modulation of codon usage
(Turun yliopisto, 2020-05-15)
Recombinant binding proteins are becoming increasingly important in various applications, including diagnostics, basic research, and therapeutics. Therefore, the demand for recombinant binding proteins will increase in ...
Rapid diagnostic tests for resource-poor areas
(Turun yliopisto, 2019-11-15)
Rapid diagnostic tests, such as lateral flow immunoassays may enable diagnostics in resource poor areas, thus improving health outcomes by helping to control and eliminate infectious diseases. This thesis focuses on the ...
Next Generation Sequencing As Assisting Tool In Protein Engineering And The Development Of A Hierarchical Dual-Index Barcoding Strategy For Screening Binder Proteins By Next Generation Sequencing
(2020-05-05)
Combinatorial gene libraries are collections of randomized DNA sequences which code, for e.g., antigen binding fragments of antibodies (Fabs). These libraries are typically enriched with molecular display methods, e.g. ...
Development of a fast, automated antigen detection test for Chlamydia trachomatis
(2020-05-07)
In recent years, there has been a global increase in the incidence of sexually transmitted infections (STIs) with Chlamydia trachomatis and Neisseria gonorrhoeae being the most frequent bacterial STIs. With an estimate of ...
Immuno-qPCR analysis and immunogenicity assessment of next generation oligonucleotides and their targeted delivery molecules
(2020-10-12)
Biotherapeutic products (BTPs) are therapeutics often made by genetically engineered organisms such as living bacterial or mammalian cells. BTPs enable effective and targeted treatment for several chronic and life-threatening ...