Screening fungal lectin activities from mushroom extracts with special reference on the Inocybe lectin and its specificity
Pirhonen, Iiro (2019-05-24)
Screening fungal lectin activities from mushroom extracts with special reference on the Inocybe lectin and its specificity
(24.05.2019)
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
avoin
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2019052817603
https://urn.fi/URN:NBN:fi-fe2019052817603
Tiivistelmä
The aim of this study was to screen mushroom extracts in search of lectin activities. This was carried out by homogenizing total amount of 129 mushroom samples and measuring their hemagglutination and hemolysis when mixed with human erythrocytes.
One fungal species, Inocybe, was studied more carefully. The second aim of the study included purifying Inocybe lectin by affinity chromatography.
The third aim of the study was to determine Inocybe´s specificity. The specificity was studied with carbohydrates and glycoproteins mixed with both purified Inocybe lectin and Inocybe total lysate. The mixes were combined with human erythrocytes and the lectin´s specific adhesion properties were observed in round bottomed microtiter plates.
The screening phase revealed new lectin activities never described before in addition to some already known. Also, the hemagglutination assay did well on determining specific lectin properties of Inocybe although their lectin genes remain to be identified. However, fundamental research is always needed when creating new biochemical applications.
One fungal species, Inocybe, was studied more carefully. The second aim of the study included purifying Inocybe lectin by affinity chromatography.
The third aim of the study was to determine Inocybe´s specificity. The specificity was studied with carbohydrates and glycoproteins mixed with both purified Inocybe lectin and Inocybe total lysate. The mixes were combined with human erythrocytes and the lectin´s specific adhesion properties were observed in round bottomed microtiter plates.
The screening phase revealed new lectin activities never described before in addition to some already known. Also, the hemagglutination assay did well on determining specific lectin properties of Inocybe although their lectin genes remain to be identified. However, fundamental research is always needed when creating new biochemical applications.