Research of protective effect of dietary fatty acids on the acute kidney injury and related mechanism
Li, Jiaqi (2019-06-28)
Research of protective effect of dietary fatty acids on the acute kidney injury and related mechanism
Li, Jiaqi
(28.06.2019)
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
suljettu
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2019071123032
https://urn.fi/URN:NBN:fi-fe2019071123032
Tiivistelmä
Rhabdomyolysis induced acute kidney injury (Rha-AKI) is renal damage caused by the products from broken muscle tissue. Folic acid induced acute kidney injury (FA-AKI) is associated with acute accumulation of folate crystals in renal tubules. The two AKI are both related to ferroptosis, which is a new way of cell death and manifested as iron dependence and lipid peroxidation. Lipid is essential to renal cells, but no research was performed to explore the effects of dietary fatty acids on these two AKI. Therefore, the research was conducted to investigate the impacts of fatty acids on AKI and the role of ferroptosis.
Rha-AKI and FA-AKI models were set in mice. Fish oil, camellia seed oil and lard were added to the mice feed as dietary supplement for docosahexaenoic acid (DHA), oleic acid (OA) and saturated fatty acid (SFA). The effects were analyzed from damage of renal function, renal histological change and change of blood lipid level. Inflammatory response and cell death were explored as well. An in vitro model was established with HK-2 cell line. Myoglobin and ferroptosis inducers were applied to investigate cytotoxicity by MTT assay. Anion probe DHE was used to reflect the accumulation of intracellular reactive oxygen species (ROS).
The research proved that DHA protected kidney damage in Rha-AKI, but promoted the damage in FA-AKI. The main reason was that DHA mainly played an anti-inflammatory role in Rha-AKI whereas in FA-AKI, the effect of aggravating cell death predominated. The research provided a new view for people who have daily supplement of DHA and also give some suggestions for the prevention and treatment of AKI.
Rha-AKI and FA-AKI models were set in mice. Fish oil, camellia seed oil and lard were added to the mice feed as dietary supplement for docosahexaenoic acid (DHA), oleic acid (OA) and saturated fatty acid (SFA). The effects were analyzed from damage of renal function, renal histological change and change of blood lipid level. Inflammatory response and cell death were explored as well. An in vitro model was established with HK-2 cell line. Myoglobin and ferroptosis inducers were applied to investigate cytotoxicity by MTT assay. Anion probe DHE was used to reflect the accumulation of intracellular reactive oxygen species (ROS).
The research proved that DHA protected kidney damage in Rha-AKI, but promoted the damage in FA-AKI. The main reason was that DHA mainly played an anti-inflammatory role in Rha-AKI whereas in FA-AKI, the effect of aggravating cell death predominated. The research provided a new view for people who have daily supplement of DHA and also give some suggestions for the prevention and treatment of AKI.