SYNTHESIS OF MESOPOPROUS SILICA NANOPARTICLES USING DROPLET MICROFLUIDICS
Tokic, Dado (2020-07-29)
SYNTHESIS OF MESOPOPROUS SILICA NANOPARTICLES USING DROPLET MICROFLUIDICS
(29.07.2020)
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
avoin
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2020101383992
https://urn.fi/URN:NBN:fi-fe2020101383992
Tiivistelmä
Introduction: Nanoparticles are small particles within nanoscale levels. Their size goes up to few hundred nanometers, although some sources state that nanoparticles are up to 100nm in diameter. Structure and function can vary between different nanoparticle models, each depending on physical properties of particles as well as way of their production. Each particle represents one functional unit. One of the most used type of nanoparticle is mesoporous silica nanoparticle. This is round shaped nanoparticle made of mesoporous silica, which makes it widespread when it comes to the drug loading. Despite the thing that nanomedicine sounds perfect in theory and brings enormous potential into targeted drug delivery, in real life it is hard to predict its behavior in vitro, and especially in vivo.
Objectives: One way to enhance synthesis of MSNs and improve its efficiency is use of microfluidic chips and techniques. Microfluidic chip brings opportunity to manipulate different fluid flow in order to synthesize nanoparticles inside a picoliter volume droplets. The first objective is to optimize the microfluidic system in order to create stable droplets in order to synthesize MSNs inside a droplet. Second objective was to wash out the sample and measure the particles in order to check their size. Third objective was to image the particles with TEM (Transmission Electron Microscope) to see if their shape and size are suitable for drug loading, coating and similar manipulations, as well as establishing the protocol for the full process.
Methods: Optimization is conducted by adjusting different flow rates and concentration of CTAB, TEOS, SPAN65 which are surfactants, precipitates and solvents. The main goal of optimization is to create stable fluid flow and stable droplets. Optimization process is monitored in real time with high speed microscope camera. Fluid flows of each substance were adjusted with fluid flow pumps. The main goal is to create a stable flow thus having a stable droplets in a sample. After the formation and collection of stable droplets, the sample is centrifuged and washed with ammonium hydroxide and ethanol solution for three times. After washing sample should be taken to Zetasizer, in order to measure particle size. If the sample is within certain nanometer range, it will be stored and imaged with Transmission electron microscope. Obtained images will be prone to image analysis with imageJ, from which data analysis will be obtained as well.
Keywords: Mesoporous Silica Nanoparticle (MSN), Transmission Electron Microscope (TEM), Cetyl Trimethyl Ammonium Bromide (CTAB), Tetra Orto Silicate (TEOS)
Objectives: One way to enhance synthesis of MSNs and improve its efficiency is use of microfluidic chips and techniques. Microfluidic chip brings opportunity to manipulate different fluid flow in order to synthesize nanoparticles inside a picoliter volume droplets. The first objective is to optimize the microfluidic system in order to create stable droplets in order to synthesize MSNs inside a droplet. Second objective was to wash out the sample and measure the particles in order to check their size. Third objective was to image the particles with TEM (Transmission Electron Microscope) to see if their shape and size are suitable for drug loading, coating and similar manipulations, as well as establishing the protocol for the full process.
Methods: Optimization is conducted by adjusting different flow rates and concentration of CTAB, TEOS, SPAN65 which are surfactants, precipitates and solvents. The main goal of optimization is to create stable fluid flow and stable droplets. Optimization process is monitored in real time with high speed microscope camera. Fluid flows of each substance were adjusted with fluid flow pumps. The main goal is to create a stable flow thus having a stable droplets in a sample. After the formation and collection of stable droplets, the sample is centrifuged and washed with ammonium hydroxide and ethanol solution for three times. After washing sample should be taken to Zetasizer, in order to measure particle size. If the sample is within certain nanometer range, it will be stored and imaged with Transmission electron microscope. Obtained images will be prone to image analysis with imageJ, from which data analysis will be obtained as well.
Keywords: Mesoporous Silica Nanoparticle (MSN), Transmission Electron Microscope (TEM), Cetyl Trimethyl Ammonium Bromide (CTAB), Tetra Orto Silicate (TEOS)