dc.contributor.author | Reinholm, Arttu | |
dc.date.accessioned | 2021-11-23T22:01:24Z | |
dc.date.available | 2021-11-23T22:01:24Z | |
dc.date.issued | 2021-10-18 | |
dc.identifier.uri | https://www.utupub.fi/handle/10024/152839 | |
dc.description.abstract | Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) from the Coronaviridae family is an enveloped, spherical virus with a 30 kb long non-segmented positive single-stranded RNA genome, which encodes for 16 non-structural, four structural, and eleven accessory proteins. It was first discovered in 2019 in Wuhan, Hubei province, China, from a patient suffering from pneumonia caused by a previously unknown virus. SARS-CoV-2 rapidly spread throughout Wuhan, after which it spread around the world, leading to the current pandemic. The common symptoms caused by SARS-CoV-2 are fever, cough, dyspnea, and myalgia, similar to other human-infecting coronaviruses such as SARS-CoV and MERS-CoV, but less severe. SARS-CoV-2 has been shown to inhibit the RIG-I pathway, disrupting the production of type I and III interferons, and to evade host innate immune response.
In this study, I analyzed whether some SARS-CoV-2 proteins have an ability to inhibit the production of type III interferon by interfering with the RIG-I pathway. Additionally, I checked if the ORF9B accessory protein was immunogenic. To accomplish this, I cloned the genes of interest into mammalian expression vectors, which were then transfected to HEK-293 cells along with a constitutively active form of RIG-I and IFN-1 promoter luciferase reporter plasmid. Structural proteins were incapable of inhibiting RIG-I induced activation of type III interferon promoter, while nonstructural proteins Nsp1, Nsp6, and Nsp13 showed clear inhibitory activity. ORF9B was found not to be immunogenic. | |
dc.format.extent | 61 | |
dc.language.iso | eng | |
dc.rights | fi=Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.|en=This publication is copyrighted. You may download, display and print it for Your own personal use. Commercial use is prohibited.| | |
dc.subject | RIG-I, SARS-CoV-2, nonstructural protein, luciferase, western blotting, interferon | |
dc.title | Inhibitory effects of SARS-CoV-2 nonstructural proteins on the RIG-I signaling pathway | |
dc.type.ontasot | fi=Pro gradu -tutkielma|en=Master's thesis| | |
dc.rights.accessrights | avoin | |
dc.identifier.urn | URN:NBN:fi-fe2021112356455 | |
dc.contributor.faculty | fi=Teknillinen tiedekunta|en=Faculty of Technology| | |
dc.contributor.studysubject | fi=Biokemia|en=Biochemistry| | |
dc.contributor.department | fi=Bioteknologian laitos|en=Department of Life Technologies| | |