dc.contributor.author | de Bijl J.J.J. | |
dc.contributor.author | Tiemeijer L.A. | |
dc.contributor.author | Stassen O.M.J.A. | |
dc.contributor.author | Bentley K. | |
dc.contributor.author | Ahlberg J.J. | |
dc.contributor.author | Chen C.S. | |
dc.contributor.author | Bouten C.V.C. | |
dc.contributor.author | Sahlgren C.M. | |
dc.contributor.author | Ristori T. | |
dc.date.accessioned | 2022-10-27T12:17:03Z | |
dc.date.available | 2022-10-27T12:17:03Z | |
dc.identifier.uri | https://www.utupub.fi/handle/10024/157537 | |
dc.description.abstract | <p>Spatial regulation of angiogenesis is important for the generation of functional<br>engineered vasculature in regenerative medicine. The Notch ligands Jag1 and<br>Dll4 show distinct expression patterns in endothelial cells and, respectively, pro-<br>mote and inhibit endothelial sprouting. Therefore, patterns of Notch ligands may<br>be utilized to spatially control sprouting, but their potential and the underlying<br>mechanisms of action are unclear. Here, we coupled in vitro and in silico models<br>to analyze the ability of micropatterned Jag1 and Dll4 ligands to spatially control<br>endothelial sprouting. Dll4 patterns, but not Jag1 patterns, elicited spatial con-<br>trol. Computational simulations of the underlying signaling dynamics suggest<br>that different timing of Notch activation by Jag1 and Dll4 underlie their distinct<br>ability to spatially control sprouting. Hence, Dll4 patterns efficiently direct the<br>sprouts, whereas longer exposure to Jag1 patterns is required to achieve spatial<br>control. These insights in sprouting regulation offer therapeutic handles for<br>spatial regulation of angiogenesis.<br></p> | |
dc.language.iso | en | |
dc.publisher | Elsevier Inc. | |
dc.title | Engineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sprouting | |
dc.identifier.url | https://doi.org/10.1016/j.isci.2022.104306 | |
dc.identifier.urn | URN:NBN:fi-fe2022081153839 | |
dc.relation.volume | 25 | |
dc.contributor.organization | fi=Turun biotiedekeskus|en=Turku Bioscience Centre| | |
dc.contributor.organization-code | 2609201 | |
dc.converis.publication-id | 175641460 | |
dc.converis.url | https://research.utu.fi/converis/portal/Publication/175641460 | |
dc.identifier.eissn | 2589-0042 | |
dc.identifier.jour-issn | 2589-0042 | |
dc.okm.affiliatedauthor | Stassen, Oscar | |
dc.okm.discipline | 3111 Biomedicine | en_GB |
dc.okm.discipline | 3111 Biolääketieteet | fi_FI |
dc.okm.internationalcopublication | international co-publication | |
dc.okm.internationality | International publication | |
dc.okm.type | Journal article | |
dc.publisher.country | Alankomaat | fi_FI |
dc.publisher.country | Netherlands | en_GB |
dc.publisher.country-code | NL | |
dc.relation.doi | 10.1016/j.isci.2022.104306 | |
dc.relation.ispartofjournal | iScience | |
dc.relation.issue | 5 | |
dc.year.issued | 2022 | |