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Engineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sprouting

de Bijl J.J.J.; Tiemeijer L.A.; Stassen O.M.J.A.; Bentley K.; Ahlberg J.J.; Chen C.S.; Bouten C.V.C.; Sahlgren C.M.; Ristori T.

dc.contributor.authorde Bijl J.J.J.
dc.contributor.authorTiemeijer L.A.
dc.contributor.authorStassen O.M.J.A.
dc.contributor.authorBentley K.
dc.contributor.authorAhlberg J.J.
dc.contributor.authorChen C.S.
dc.contributor.authorBouten C.V.C.
dc.contributor.authorSahlgren C.M.
dc.contributor.authorRistori T.
dc.date.accessioned2022-10-27T12:17:03Z
dc.date.available2022-10-27T12:17:03Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/157537
dc.description.abstract<p>Spatial regulation of angiogenesis is important for the generation of functional<br>engineered vasculature in regenerative medicine. The Notch ligands Jag1 and<br>Dll4 show distinct expression patterns in endothelial cells and, respectively, pro-<br>mote and inhibit endothelial sprouting. Therefore, patterns of Notch ligands may<br>be utilized to spatially control sprouting, but their potential and the underlying<br>mechanisms of action are unclear. Here, we coupled in vitro and in silico models<br>to analyze the ability of micropatterned Jag1 and Dll4 ligands to spatially control<br>endothelial sprouting. Dll4 patterns, but not Jag1 patterns, elicited spatial con-<br>trol. Computational simulations of the underlying signaling dynamics suggest<br>that different timing of Notch activation by Jag1 and Dll4 underlie their distinct<br>ability to spatially control sprouting. Hence, Dll4 patterns efficiently direct the<br>sprouts, whereas longer exposure to Jag1 patterns is required to achieve spatial<br>control. These insights in sprouting regulation offer therapeutic handles for<br>spatial regulation of angiogenesis.<br></p>
dc.language.isoen
dc.publisherElsevier Inc.
dc.titleEngineered patterns of Notch ligands Jag1 and Dll4 elicit differential spatial control of endothelial sprouting
dc.identifier.urlhttps://doi.org/10.1016/j.isci.2022.104306
dc.identifier.urnURN:NBN:fi-fe2022081153839
dc.relation.volume25
dc.contributor.organizationfi=Turun biotiedekeskus|en=Turku Bioscience Centre|
dc.contributor.organization-code2609201
dc.converis.publication-id175641460
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/175641460
dc.identifier.eissn2589-0042
dc.identifier.jour-issn2589-0042
dc.okm.affiliatedauthorStassen, Oscar
dc.okm.discipline3111 Biomedicineen_GB
dc.okm.discipline3111 Biolääketieteetfi_FI
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countryAlankomaatfi_FI
dc.publisher.countryNetherlandsen_GB
dc.publisher.country-codeNL
dc.relation.doi10.1016/j.isci.2022.104306
dc.relation.ispartofjournaliScience
dc.relation.issue5
dc.year.issued2022


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