Influence of the Assembly State on the Functionality of a Supramolecular Jagged1-Mimicking Peptide Additive
Cecilia M. Sahlgren; Maaike J. G. Schotman; Patricia Y. W. Dankers; Matilde Putti; Oscar M. J. A. Stassen
Influence of the Assembly State on the Functionality of a Supramolecular Jagged1-Mimicking Peptide Additive
Cecilia M. Sahlgren
Maaike J. G. Schotman
Patricia Y. W. Dankers
Matilde Putti
Oscar M. J. A. Stassen
AMER CHEMICAL SOC
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042823144
https://urn.fi/URN:NBN:fi-fe2021042823144
Tiivistelmä
Expanding the bioactivation toolbox of supramolecular materials is of utmost relevance for their broad applicability in regenerative medicines. This study explores the functionality of a peptide mimic of the Notch ligand Jagged1 in a supramolecular system that is based on hydrogen bonding ureido-pyrimidinone (UPy) units. The functionality of the peptide is studied when formulated as an additive in a supramolecular solid material and as a self-assembled system in solution. UPy conjugation of the DSLJAG1 peptide sequence allows for the supramolecular functionalization of UPy-modified polycaprolactone, an elastomeric material, with UPy-DSLJAG1. Surface presentation of the UPy-DSLJAG1 peptide was confirmed by atomic force microscopy and X-ray photoelectron spectroscopy analyses, but no enhancement of Notch activity was detected in cells presenting Notch1 and Notch3 receptors. Nevertheless, a significant increase in Notch-signaling activity was observed when DSLJAG1 peptides were administered in the soluble form, indicating that the activity of DSLJAG1 is preserved after UPy functionalization but not after immobilization on a supramolecular solid material. Interestingly, an enhanced activity in solution of the UPy conjugate was detected compared with the unconjugated DSLJAG1 peptide, suggesting that the self-assembly of supramolecular aggregates in solution ameliorates the functionality of the molecules in a biological context.
Kokoelmat
- Rinnakkaistallenteet [19207]