Näytä suppeat kuvailutiedot

Fiber depth, column coating and extraction time are major contributors in the headspace solid-phase microextraction–gas chromatography analysis of Nordic wild mushrooms

Linderborg K.; Aisala H.; Sandell M.

dc.contributor.authorLinderborg K.
dc.contributor.authorAisala H.
dc.contributor.authorSandell M.
dc.date.accessioned2022-10-27T12:23:58Z
dc.date.available2022-10-27T12:23:58Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/158339
dc.description.abstract<p>The aims of this research were to systematically study how extraction, desorption and gas chromatography (GC) parameters affect the volatiles composition of mushrooms in headspace solid-phase microextraction (HS-SPME) analysis. The study was carried out both with reference compounds and with Cantharellus cibarius Fr. mushroom sample. The experiments were carried out with full-factorial multivariate designs. In the desorption studies, high fiber depth in the GC injector port instead of desorption time or temperature was the most important variable for maximizing peak areas. This could be a function related to the temperature gradient inside the injector as well as a faster transfer of volatiles to the column. Out of the tested fibers, divinylbenzene/carboxen/polydimethylsiloxane (DVB/Car/PDMS) was the most suitable coating for mushroom volatiles. Additionally, extraction time of 45 vs 30 min had a high effect, while doubling the sample volume had a minimal effect. 21 volatiles were identified in C. cibarius. Hexanal and 1-octen-3-ol were the most abundant volatile compounds. Overall, aldehydes and unsaturated C6–C10 alcohols and aldehydes were the most abundant compound groups. This study demonstrated that despite the wide adaptation and history of SPME, fiber injection depth, fiber coating and extraction time are important factors that should still be carefully tested as a part of method development for mushroom-type matrices. Finally, the results of this study show that it is important to look at the extracted gas chromatogram as a whole instead of focusing on a few peaks of interest.<br /></p>
dc.language.isoen
dc.publisherSpringer Verlag
dc.titleFiber depth, column coating and extraction time are major contributors in the headspace solid-phase microextraction–gas chromatography analysis of Nordic wild mushrooms
dc.identifier.urnURN:NBN:fi-fe2021042718130
dc.relation.volume244
dc.contributor.organizationfi=funktion. elint. kehittämiskeskus (yhteiset)|en=Functional Foods Forum|
dc.contributor.organizationfi=PÄÄT Elintarvikekemia ja elintarvikekehitys|en=PÄÄT Food Chemistry and Food Development|
dc.contributor.organization-code2606204
dc.contributor.organization-code2607020
dc.converis.publication-id28743888
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/28743888
dc.format.pagerange850
dc.format.pagerange841
dc.identifier.eissn1438-2385
dc.identifier.jour-issn1438-2377
dc.okm.affiliatedauthorLinderborg, Kaisa
dc.okm.affiliatedauthorSandell, Mari
dc.okm.affiliatedauthorAisala, Heikki
dc.okm.discipline215 Teknillinen kemia, kemian prosessitekniikkafi_FI
dc.okm.discipline215 Chemical engineeringen_GB
dc.okm.discipline116 Kemiafi_FI
dc.okm.discipline116 Chemical sciencesen_GB
dc.okm.internationalcopublicationnot an international co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countryGermanyen_GB
dc.publisher.countrySaksafi_FI
dc.publisher.country-codeDE
dc.relation.doi10.1007/s00217-017-3005-0
dc.relation.ispartofjournalEuropean Food Research and Technology
dc.relation.issue5
dc.year.issued2018


Aineistoon kuuluvat tiedostot

Thumbnail

Aineisto kuuluu seuraaviin kokoelmiin

Näytä suppeat kuvailutiedot