Isolation of intact and pure chloroplasts from leaves of Arabidopsis thaliana plants acclimated to low irradiance for studies on Rubisco regulation
Jackowski G; Grabsztunowicz M
Isolation of intact and pure chloroplasts from leaves of Arabidopsis thaliana plants acclimated to low irradiance for studies on Rubisco regulation
Jackowski G
Grabsztunowicz M
POLSKIE TOWARZYSTWO BOTANICZNE
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042720348
https://urn.fi/URN:NBN:fi-fe2021042720348
Tiivistelmä
A protocol is presented for low-cost and fast isolation of intact and pure chloroplasts from leaves of plants acclimated to low irradiance. The protocol is based on a differential centrifugation of cleared leaf homogenate and omits a centrifugation on Percoll gradient step. The intactness and purity of the chloroplasts isolated from leaves of low irradiance-acclimated plants by using this protocol (confirmed by phase contrast microscopy as well as enzymatic and immunological approaches) allows plausible studies on low irradiance-dependent Rubisco regulation.
Kokoelmat
- Rinnakkaistallenteet [19207]