Näytä suppeat kuvailutiedot

Biocontrol of Fusarium graminearum, a Causal Agent of Fusarium Head Blight of Wheat, and Deoxynivalenol Accumulation: From In Vitro to In Planta

Yli-Mattila Tapani; Abbas Asmaa

dc.contributor.authorYli-Mattila Tapani
dc.contributor.authorAbbas Asmaa
dc.date.accessioned2022-10-28T12:25:55Z
dc.date.available2022-10-28T12:25:55Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/159432
dc.description.abstract<p>Crop diseases caused by <i>Fusarium graminearum</i> threaten crop production in both commercial and smallholder farming.<i> F. graminearum</i> produces deoxynivalenol mycotoxin, which is stable during food and feed processing. Therefore, the best way to prevent the sporulation of pathogens is to develop new prevention strategies. Plant-based pesticides, i.e., natural fungicides, have recently gained interest in crop protection as alternatives to synthetic fungicides. Herein we show that treatment with the methanolic extract of medicinal plant <i>Zanthoxylum bungeanum</i> (M20 extract), decreased <i>F. graminearum</i> growth and abrogated DON production. The <i>F. graminearum</i> DNA levels were monitored by a quantitative TaqMan real-time PCR, while DON accumulation was assessed by HPLC quantification. This M20 extract was mainly composed of four flavonoids: quercetin, epicatechin, kaempferol-3-O-rhamnoside, and hyperoside. The in vitro bioassay, which measured the percent inhibition of fungal growth, showed that co-inoculation of four <i>F. graminearum</i> strains with the M20 extract inhibited the fungal growth up to 48.5%. After biocontrol treatments,<i> F. graminearum</i> DNA level was reduced up to 85.5% compared to that of wheat heads, which received F. graminearum mixture only. Moreover, DON production was decreased in wheat heads by 73% after biocontrol treatment; meanwhile in wheat heads inoculated with F. graminearum conidia, an average of 2.263 ± 0.8 mg/kg DON was detected. Overall, this study is a successful case from in vitro research to in planta, giving useful information for wheat protection against F. graminearum responsible for Fusarium Head Blight and DON accumulation in grains. Further studies are needed to study the mechanism by which M20 extract inhibited the DON production and what changes happened to the DON biosynthetic pathway genes.<br></p>
dc.language.isoen
dc.publisherMDPI
dc.titleBiocontrol of Fusarium graminearum, a Causal Agent of Fusarium Head Blight of Wheat, and Deoxynivalenol Accumulation: From In Vitro to In Planta
dc.identifier.urlhttps://doi.org/10.3390/toxins14050299
dc.identifier.urnURN:NBN:fi-fe2022081154024
dc.relation.volume14
dc.contributor.organizationfi=mat.-luonn.t. tdk yhteiset|en=Mat.-luonn.t. tdk yhteiset|
dc.contributor.organizationfi=biokemia|en=Biokemia|
dc.contributor.organizationfi=molekulaarinen kasvibiologia|en=Molekulaarinen kasvibiologia|
dc.contributor.organization-code2606000
dc.contributor.organization-code2610101
dc.converis.publication-id175677604
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/175677604
dc.identifier.eissn2072-6651
dc.identifier.jour-issn2072-6651
dc.okm.affiliatedauthorYli-Mattila, Tapani
dc.okm.affiliatedauthorAbbas, Asmaa
dc.okm.affiliatedauthorDataimport, 2610104 molekulaarinen kasvibiologia
dc.okm.discipline1182 Biochemistry, cell and molecular biologyen_GB
dc.okm.discipline1183 Plant biology, microbiology, virologyen_GB
dc.okm.discipline1182 Biokemia, solu- ja molekyylibiologiafi_FI
dc.okm.discipline1183 Kasvibiologia, mikrobiologia, virologiafi_FI
dc.okm.internationalcopublicationnot an international co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countrySwitzerlanden_GB
dc.publisher.countrySveitsifi_FI
dc.publisher.country-codeCH
dc.relation.articlenumber299
dc.relation.doi10.3390/toxins14050299
dc.relation.ispartofjournalToxins
dc.relation.issue5
dc.year.issued2022


Aineistoon kuuluvat tiedostot

Thumbnail

Aineisto kuuluu seuraaviin kokoelmiin

Näytä suppeat kuvailutiedot