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BLIMP-1 is insufficient to induce antibody secretion in the absence of IRF4 in DT40 cells

Budzynska PM; Lassila O; Kyläniemi MK; Nera KP; Alinikula J

dc.contributor.authorBudzynska PM
dc.contributor.authorLassila O
dc.contributor.authorKyläniemi MK
dc.contributor.authorNera KP
dc.contributor.authorAlinikula J
dc.date.accessioned2022-10-28T12:26:01Z
dc.date.available2022-10-28T12:26:01Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/159445
dc.description.abstractDifferentiation of B cells into antibody-secreting cells (ASCs), plasmablasts and plasma cells is regulated by a network of transcription factors. Within this network, factors including PAX5 and BCL6 prevent ASC differentiation and maintain the B cell phenotype. In contrast, BLIMP-1 and high IRF4 expression promote plasma cell differentiation. BLIMP-1 is thought to induce immunoglobulin secretion, whereas IRF4 is needed for the survival of ASCs. The role of IRF4 in the regulation of antibody secretion has remained controversial. To study the role of IRF4 in the regulation of antibody secretion, we have created a double knockout (DKO) DT40 B cell line deficient in both IRF4 and BCL6. Although BCL6-deficient DT40 B cell line had upregulated BLIMP-1 expression and secreted antibodies, the DKO cell line did not. Even enforced BLIMP-1 expression in DKO cells or IRF4-deficient cells could not induce IgM secretion while in WT DT40 cells, it could. However, enforced IRF4 expression in DKO cells induced strong IgM secretion. Our findings support a model where IRF4 expression in addition to BLIMP-1 expression is required to induce robust antibody secretion.
dc.language.isoen
dc.publisherWILEY
dc.titleBLIMP-1 is insufficient to induce antibody secretion in the absence of IRF4 in DT40 cells
dc.identifier.urnURN:NBN:fi-fe2021042718921
dc.relation.volume87
dc.contributor.organizationfi=tyks, vsshp|en=tyks, vsshp|
dc.contributor.organizationfi=biolääketieteen laitos, yhteiset|en=Institute of Biomedicine|
dc.contributor.organization-code2607100
dc.converis.publication-id30384308
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/30384308
dc.identifier.eissn1365-3083
dc.identifier.jour-issn0300-9475
dc.okm.affiliatedauthorDataimport, tyks, vsshp
dc.okm.affiliatedauthorNera, Kalle-Pekka
dc.okm.affiliatedauthorLassila, Olli
dc.okm.affiliatedauthorBudzynska, Paulina
dc.okm.affiliatedauthorAlinikula, Jukka
dc.okm.affiliatedauthorKyläniemi, Minna
dc.okm.discipline3111 Biolääketieteetfi_FI
dc.okm.discipline3111 Biomedicineen_GB
dc.okm.internationalcopublicationnot an international co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countryUnited Kingdomen_GB
dc.publisher.countryBritanniafi_FI
dc.publisher.country-codeGB
dc.relation.articlenumberUNSP e12646
dc.relation.doi10.1111/sji.12646
dc.relation.ispartofjournalScandinavian Journal of Immunology
dc.relation.issue3
dc.year.issued2018


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