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Quantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy

Virtanen L; Taimen P; Kittisopikul M; Goldman RD

dc.contributor.authorVirtanen L
dc.contributor.authorTaimen P
dc.contributor.authorKittisopikul M
dc.contributor.authorGoldman RD
dc.date.accessioned2022-10-28T12:27:32Z
dc.date.available2022-10-28T12:27:32Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/159637
dc.description.abstractThe nuclear lamina consists of a dense fibrous meshwork of nuclear lamins, Type V intermediate filaments, and is similar to 14 nm thick according to recent cryo-electron tomography studies. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole nucleus. We review quantitative approaches to analyze the imaging data of the nuclear lamina as acquired by structured illumination microscopy (SIM) and single molecule localization microscopy (SMLM), as well as the requisite cell preparation techniques. In particular, we discuss the application of steerable filters and graph-based methods to segment the structure of the four mammalian lamin isoforms (A, C, B1, and B2) and extract quantitative information.
dc.language.isoen
dc.publisherMDPI
dc.titleQuantitative Analysis of Nuclear Lamins Imaged by Super-Resolution Light Microscopy
dc.identifier.urlhttps://www.mdpi.com/2073-4409/8/4/361/htm
dc.identifier.urnURN:NBN:fi-fe2021042824644
dc.relation.volume8
dc.contributor.organizationfi=biolääketieteen laitos, yhteiset|en=Institute of Biomedicine|
dc.contributor.organizationfi=tyks, vsshp|en=tyks, vsshp|
dc.contributor.organization-code2607100
dc.converis.publication-id41296377
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/41296377
dc.identifier.eissn2073-4409
dc.identifier.jour-issn2073-4409
dc.okm.affiliatedauthorDataimport, tyks, vsshp
dc.okm.affiliatedauthorTaimen, Pekka
dc.okm.affiliatedauthorVirtanen, Laura
dc.okm.discipline3111 Biolääketieteetfi_FI
dc.okm.discipline3111 Biomedicineen_GB
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeB1 Non-refereed journal articles
dc.publisher.countrySveitsifi_FI
dc.publisher.countrySwitzerlanden_GB
dc.publisher.country-codeCH
dc.relation.articlenumber361
dc.relation.doi10.3390/cells8040361
dc.relation.ispartofjournalCells
dc.relation.issue4
dc.year.issued2019


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