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Matrix metalloproteinase-7 in periodontitis with type 2 diabetes mellitus

L. Häkkinen; F. Caglayan; V.‐J. Uitto; B.‐H. Neves de Oliveira; F. Zeidán‐Chuliá; G. Güncü; D. Yilmaz; U. K. Gürsoy; E. Könönen

dc.contributor.authorL. Häkkinen
dc.contributor.authorF. Caglayan
dc.contributor.authorV.‐J. Uitto
dc.contributor.authorB.‐H. Neves de Oliveira
dc.contributor.authorF. Zeidán‐Chuliá
dc.contributor.authorG. Güncü
dc.contributor.authorD. Yilmaz
dc.contributor.authorU. K. Gürsoy
dc.contributor.authorE. Könönen
dc.date.accessioned2022-10-28T12:30:09Z
dc.date.available2022-10-28T12:30:09Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/159962
dc.description.abstract<p>Background and Objective: Elevated levels of matrix metalloproteinase‐7 (MMP7) have been observed in serum samples of subjects with type 2 diabetes mellitus (T2DM) and in gingival tissues of subjects with periodontitis. The aim of the present study was to collect in vivo and in silico evidence on the role of MMP7 in the interplay between T2DM and generalized periodontitis (GP).</p><p>Material and Methods: The extent of MMP7 expression and localization were immunohistochemically analyzed in gingival tissues of patients with GP with T2DM (T2DM/GP, n = 11), systemically healthy patients with GP (n = 7), and systemically and periodontally healthy controls (n = 11). An in silico network model was built to determine the interactions between MMP7 and T2DM pathways. Regulation of neutrophil transmigration by MMP7 was analyzed in a knock‐out mice model.</p><p>Results: In human gingival tissues, the proportion of cells with robust MMP7 expression was elevated in patients with T2DM/GP in comparison to controls (P = .014). According to the in silico analysis, “hydroxyl radical” and “hydrogen peroxide” compounds were among the most central nodes of the network, and were within the shortest paths connecting “glucose” to “MMP7.” In MMP7 knock‐out mice, an intense accumulation of neutrophils was observed in the gingival epithelium as compared to wild‐type mice (P = .0001). </p><p>Conclusion: Elevated MMP7 expression in gingival tissues of patients with T2DM/GP is related to the activation of reactive oxygen species by hyperglycemia. Suppression of MMP7 expression results in impaired neutrophil transmigration in gingiva.<br /></p>
dc.language.isoen
dc.publisherBlackwell Munksgaard
dc.titleMatrix metalloproteinase-7 in periodontitis with type 2 diabetes mellitus
dc.identifier.urnURN:NBN:fi-fe2021042719664
dc.relation.volume53
dc.contributor.organizationfi=hammaslääketieteen laitos yhteiset|en=Institute of Dentistry|
dc.contributor.organization-code2607500
dc.converis.publication-id35693679
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/35693679
dc.format.pagerange916
dc.format.pagerange923
dc.identifier.eissn1600-0765
dc.identifier.jour-issn0022-3484
dc.okm.affiliatedauthorGursoy, Ulvi
dc.okm.affiliatedauthorZeidan Chulia, Fares
dc.okm.affiliatedauthorKönönen, Eija
dc.okm.discipline313 Dentistryen_GB
dc.okm.discipline313 Hammaslääketieteetfi_FI
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countryTanskafi_FI
dc.publisher.countryDenmarken_GB
dc.publisher.country-codeDK
dc.relation.doi10.1111/jre.12583
dc.relation.ispartofjournalJournal of Periodontal Research
dc.relation.issue5
dc.year.issued2018


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