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The interactome of the prostate-specific protein Anoctamin 7

Elina Kaikkonen; Juha-Pekka Pursiheimo; Aliisa Takala; Johanna Schleutker; Gudrun Wahlström

dc.contributor.authorElina Kaikkonen
dc.contributor.authorJuha-Pekka Pursiheimo
dc.contributor.authorAliisa Takala
dc.contributor.authorJohanna Schleutker
dc.contributor.authorGudrun Wahlström
dc.date.accessioned2022-10-28T12:38:10Z
dc.date.available2022-10-28T12:38:10Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/160951
dc.description.abstractBACKGROUND: Elevated Anoctamin 7 (ANO7) expression is associated with poor survival in prostate cancer patients.OBJECTIVE: The aim was to discover proteins that interact with ANO7 to understand its functions and regulatory mechanisms.METHODS: The proximity-dependent biotin identification (BioID) method was utilized. ANO7 fused to biotin ligase was transiently transfected into LNCaP cells, and the biotinylated proteins were collected and analysed by mass spectrometry. Four identified proteins were stained with dual fluorescent immunostaining and visualized using Stimulated emission depletion microscopy (STED).RESULTS: After bioinformatic filtering steps, 64 potentially ANO7-interacting proteins were identified and analysed with the GO enrichment analysis tool. One of the most prominently enriched cellular components was cellular vesicle. Co-localization was showed for staphylococcal nuclease and tudor domain containing 1 (SND1), heat shock protein family A (Hsp70) member 1A (HSPA1A), adaptor related protein complex 2 subunit beta 1 (AP2B1) and coatomer protein complex subunit gamma 2 (COPG2).CONCLUSIONS: This is the first study in which ANO7 interacting proteins have been identified. Although further studies are needed, the findings reported here expand our understanding of the role and regulation of ANO7 in prostate cancer cells. Furthermore, these results are likely to introduce new targets for the novel cancer therapies.
dc.language.isoen
dc.publisherIOS PRESS
dc.titleThe interactome of the prostate-specific protein Anoctamin 7
dc.identifier.urnURN:NBN:fi-fe2021042825577
dc.relation.volume28
dc.contributor.organizationfi=tyks, vsshp|en=tyks, vsshp|
dc.contributor.organizationfi=biolääketieteen laitos, yhteiset|en=Institute of Biomedicine|
dc.contributor.organization-code2607100
dc.converis.publication-id48474953
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/48474953
dc.format.pagerange100
dc.format.pagerange91
dc.identifier.eissn1875-8592
dc.identifier.jour-issn1574-0153
dc.okm.affiliatedauthorWahlström, Gudrun
dc.okm.affiliatedauthorKaikkonen, Elina
dc.okm.affiliatedauthorDataimport, tyks, vsshp
dc.okm.affiliatedauthorSchleutker, Johanna
dc.okm.affiliatedauthorPursiheimo, Juha
dc.okm.affiliatedauthorTakala, Aliisa
dc.okm.discipline3122 Syöpätauditfi_FI
dc.okm.discipline3111 Biomedicineen_GB
dc.okm.discipline3122 Cancersen_GB
dc.okm.discipline3111 Biolääketieteetfi_FI
dc.okm.internationalcopublicationnot an international co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countryAlankomaatfi_FI
dc.publisher.countryNetherlandsen_GB
dc.publisher.country-codeNL
dc.relation.doi10.3233/CBM-190993
dc.relation.ispartofjournalCancer Biomarkers
dc.relation.issue1
dc.year.issued2020


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