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Influence of Cell Membrane Wrapping on the Cell-Porous Silicon Nanoparticle Interactions

Joseph Ndika; Arto Urtti; Vincenzo Cerullo; Jarno Salonen; Alexandra Correia; Jonas Buck; Jacopo Chiaro; Sandro Sieber; Flavia Fontana; Ermei Mäkilä; Jouni T. Hirvonen; Hélder A. Santos; Harri Alenius; Hanna Lindstedt; Otto K. Kari

Influence of Cell Membrane Wrapping on the Cell-Porous Silicon Nanoparticle Interactions

Joseph Ndika
Arto Urtti
Vincenzo Cerullo
Jarno Salonen
Alexandra Correia
Jonas Buck
Jacopo Chiaro
Sandro Sieber
Flavia Fontana
Ermei Mäkilä
Jouni T. Hirvonen
Hélder A. Santos
Harri Alenius
Hanna Lindstedt
Otto K. Kari
Katso/Avaa
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WILEY
doi:10.1002/adhm.202000529
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Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042826113
Tiivistelmä
Biohybrid nanosystems represent the cutting-edge research in biofunctionalization of micro- and nano-systems. Their physicochemical properties bring along advantages in the circulation time, camouflaging from the phagocytes, and novel antigens. This is partially a result of the qualitative differences in the protein corona, and the preferential targeting and uptake in homologous cells. However, the effect of the cell membrane on the cellular endocytosis mechanisms and time has not been fully evaluated yet. Here, the effect is assessed by quantitative flow cytometry analysis on the endocytosis of hydrophilic, negatively charged porous silicon nanoparticles and on their membrane-coated counterparts, in the presence of chemical inhibitors of different uptake pathways. Principal component analysis is used to analyze all the data and extrapolate patterns to highlight the cell-specific differences in the endocytosis mechanisms. Furthermore, the differences in the composition of static protein corona between naked and coated particles are investigated together with how these differences affect the interaction with human macrophages. Overall, the presence of the cell membrane only influences the speed and the entity of nanoparticles association with the cells, while there is no direct effect on the endocytosis pathways, composition of protein corona, or any reduction in macrophage-mediated uptake.
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