MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines
Michael J. O. Wakelam; Gerhard Liebisch; Tze Ping Loh; Kazutaka Ikeda; Anne K. Bendt; Makoto Arita; Bo Burla; Peter J. Meikle; Markus R. Wenk; Amaury Cazenave-Gassiot; Andrej Shevchenko; Oswald Quehenberger; Federico Torta; Edward A. Dennis; Matej Orešič; Xianlin Han; Kim Ekroos; Craig E. Wheelock; Masanori Arita; Michelle K. Lin
MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines
Michael J. O. Wakelam
Gerhard Liebisch
Tze Ping Loh
Kazutaka Ikeda
Anne K. Bendt
Makoto Arita
Bo Burla
Peter J. Meikle
Markus R. Wenk
Amaury Cazenave-Gassiot
Andrej Shevchenko
Oswald Quehenberger
Federico Torta
Edward A. Dennis
Matej Orešič
Xianlin Han
Kim Ekroos
Craig E. Wheelock
Masanori Arita
Michelle K. Lin
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042719843
https://urn.fi/URN:NBN:fi-fe2021042719843
Tiivistelmä
Human blood is a self-regenerating lipid-rich biological fluid that is routinely collected in hospital settings. The inventory of lipid molecules found in blood plasma (plasma lipidome) offers insights into individual metabolism and physiology in health and disease. Disturbances in the plasma lipidome also occur in conditions that are not directly linked to lipid metabolism; therefore, plasma lipidomics based on MS is an emerging tool in an array of clinical diagnostics and disease management. However, challenges exist in the translation of such lipidomic data to clinical applications. These relate to the reproducibility, accuracy, and precision of lipid quantitation, study design, sample handling, and data sharing. This position paper emerged from a workshop that initiated a community-led process to elaborate and define a set of generally accepted guidelines for quantitative MS-based lipidomics of blood plasma or serum, with harmonization of data acquired on different instrumentation platforms across independent laboratories as an ultimate goal. We hope that other fields may benefit from and follow such a precedent.
Kokoelmat
- Rinnakkaistallenteet [19207]