Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure
Leuzinger Karoline; Feltkamp Mariet C.W.; Klapper Paul E.; Kamminga Sergio; Nielsen Xiaohui Chen; Couto Natacha; Pérez-Cataluňa Alba; van der Hoek Lia; Sayiner Arzu; Jonges Marcel; Breuer Judy; Molenkamp Richard; Van Boheemen Sander; Beer Martin; Brown Julianne R.; Madsen Tina Vasehus; Höper Dirk; Harvala Heli; Sousa Hugo; Buesa Javier; Cinek Ondrej; López-Labrador F. Xavier; Kroes Aloys C.M.; Schuurman Rob; de Vries Jutte J.C.; on behalf of the ESCV Network on Next-Generation Sequencing; Kufner Verena; Puchhammer-Stöckl Elisabeth; Fischer Nicole; Simmonds Peter; Rodríguez-Díaz Jesús; Rodriguez Christophe; Hönemann Mario; Keyaerts Els; Auvinen Eeva; Claas Eric C.J.; Susi Petri; Huber Michael; Hernandez Marta
Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure
Leuzinger Karoline
Feltkamp Mariet C.W.
Klapper Paul E.
Kamminga Sergio
Nielsen Xiaohui Chen
Couto Natacha
Pérez-Cataluňa Alba
van der Hoek Lia
Sayiner Arzu
Jonges Marcel
Breuer Judy
Molenkamp Richard
Van Boheemen Sander
Beer Martin
Brown Julianne R.
Madsen Tina Vasehus
Höper Dirk
Harvala Heli
Sousa Hugo
Buesa Javier
Cinek Ondrej
López-Labrador F. Xavier
Kroes Aloys C.M.
Schuurman Rob
de Vries Jutte J.C.; on behalf of the ESCV Network on Next-Generation Sequencing
Kufner Verena
Puchhammer-Stöckl Elisabeth
Fischer Nicole
Simmonds Peter
Rodríguez-Díaz Jesús
Rodriguez Christophe
Hönemann Mario
Keyaerts Els
Auvinen Eeva
Claas Eric C.J.
Susi Petri
Huber Michael
Hernandez Marta
Elsevier
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042820793
https://urn.fi/URN:NBN:fi-fe2021042820793
Tiivistelmä
Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.
Kokoelmat
- Rinnakkaistallenteet [19206]