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Mimics of small ribozymes utilizing a supramolecular scaffold

Lonnberg TA; Helkearo M; Jancso A; Gajda T

dc.contributor.authorLonnberg TA
dc.contributor.authorHelkearo M
dc.contributor.authorJancso A
dc.contributor.authorGajda T
dc.date.accessioned2022-10-28T13:04:50Z
dc.date.available2022-10-28T13:04:50Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/162656
dc.description.abstract<p> For elucidating the mechanism of the general acid/base catalysis of the hydrolysis of RNA phosphodiester bonds, a number of cleaving agents having two cyclen moieties tethered to a 1,3,5-triazine core have been prepared and their ability to bind and cleave uridylyl-3', 5'-uridine (UpU) studied over a wide pH range. Around neutral pH, the cleaving agents form a highly stable ternary complex with UpU and Zn-II through coordination of the uracil N3 and the cyclen nitrogen atoms to the Zn-II ions. Under conditions where the triazine core exists in the deprotonated neutral form, hydrolysis of UpU, but not of adenylyl-3',5'-adenosine (ApA), is accelerated by approximately two orders of magnitude in the presence of the cleaving agents, suggesting general base rather than metal ion catalysis. The probable mechanism of the observed catalysis and implications to understanding the general acid/base-catalyzed phosphodiester hydrolysis by ribozymes are discussed.</p>
dc.language.isoen
dc.publisherROYAL SOC CHEMISTRY
dc.titleMimics of small ribozymes utilizing a supramolecular scaffold
dc.identifier.urnURN:NBN:fi-fe2021042715106
dc.relation.volume41
dc.contributor.organizationfi=kemian laitoksen yhteiset|en=Department of Chemistry|
dc.contributor.organizationfi=orgaaninen kemia ja kemiallinen biologia|en=Laboratory of Organic Chemistry and Chemical Biology|
dc.contributor.organization-code2606303
dc.contributor.organization-code2606300
dc.converis.publication-id3195604
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/3195604
dc.format.pagerange3338
dc.format.pagerange3328
dc.identifier.jour-issn1477-9226
dc.okm.affiliatedauthorHelkearo, Mia
dc.okm.affiliatedauthorLönnberg, Tuomas
dc.okm.discipline116 Chemical sciencesen_GB
dc.okm.discipline116 Kemiafi_FI
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countryUnited Kingdomen_GB
dc.publisher.countryBritanniafi_FI
dc.publisher.country-codeGB
dc.relation.doi10.1039/c2dt10193a
dc.relation.ispartofjournalDalton Transactions
dc.relation.issue11
dc.year.issued2012


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