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Synchrotron MicroCT Reveals the Potential of the Dual Contrast Technique for Quantitative Assessment of Human Articular Cartilage Composition

Kroger H; Shaikh R; Prakash M; Toyras J; Lovric G; Saukko AEA; Joukainen A; Honkanen MKM; Grinstaff MW; Turunen MJ

dc.contributor.authorKroger H
dc.contributor.authorShaikh R
dc.contributor.authorPrakash M
dc.contributor.authorToyras J
dc.contributor.authorLovric G
dc.contributor.authorSaukko AEA
dc.contributor.authorJoukainen A
dc.contributor.authorHonkanen MKM
dc.contributor.authorGrinstaff MW
dc.contributor.authorTurunen MJ
dc.date.accessioned2022-10-28T13:05:17Z
dc.date.available2022-10-28T13:05:17Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/162702
dc.description.abstractDual contrast micro computed tomography (CT) shows potential for detecting articular cartilage degeneration. However, the performance of conventional CT systems is limited by beam hardening, low image resolution (full-body CT), and long acquisition times (conventional microCT). Therefore, to reveal the full potential of the dual contrast technique for imaging cartilage composition we employ the technique using synchrotron microCT. We hypothesize that the above-mentioned limitations are overcome with synchrotron microCT utilizing monochromatic X-ray beam and fast image acquisition. Human osteochondral samples (n = 41, four cadavers) were immersed in a contrast agent solution containing two agents (cationic CA4+ and non-ionic gadoteridol) and imaged with synchrotron microCT at an early diffusion time point (2 h) and at diffusion equilibrium (72 h) using two monochromatic X-ray energies (32 and 34 keV). The dual contrast technique enabled simultaneous determination of CA4+ (i.e., proteoglycan content) and gadoteridol (i.e., water content) partitions within cartilage. Cartilage proteoglycan content and biomechanical properties correlated significantly (0.327 < r < 0.736, p < 0.05) with CA4+ partition in superficial and middle zones at both diffusion time points. Normalization of the CA4+ partition with gadoteridol partition within the cartilage significantly (p < 0.05) improved the detection sensitivity for human osteoarthritic cartilage proteoglycan content, biomechanical properties, and overall condition (Mankin, Osteoarthritis Research Society International, and International Cartilage Repair Society grading systems). The dual energy technique combined with the dual contrast agent enables assessment of human articular cartilage proteoglycan content and biomechanical properties based on CA4+ partition determined using synchrotron microCT. Additionally, the dual contrast technique is not limited by the beam hardening artifact of conventional CT systems. (c) 2019 The Authors. Journal of Orthopaedic Research (R) published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society. J Orthop Res
dc.language.isoen
dc.publisherWILEY
dc.titleSynchrotron MicroCT Reveals the Potential of the Dual Contrast Technique for Quantitative Assessment of Human Articular Cartilage Composition
dc.identifier.url10.1002/jor.24479
dc.identifier.urnURN:NBN:fi-fe2021042821116
dc.relation.volume38
dc.contributor.organizationfi=tyks, vsshp|en=tyks, vsshp|
dc.converis.publication-id42615562
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/42615562
dc.format.pagerange563
dc.format.pagerange573
dc.identifier.eissn0736-0266
dc.identifier.jour-issn0736-0266
dc.okm.affiliatedauthorDataimport, tyks, vsshp
dc.okm.discipline3126 Kirurgia, anestesiologia, tehohoito, radiologiafi_FI
dc.okm.discipline3126 Surgery, anesthesiology, intensive care, radiologyen_GB
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countryUnited Statesen_GB
dc.publisher.countryYhdysvallat (USA)fi_FI
dc.publisher.country-codeUS
dc.publisher.placeHOBOKEN
dc.relation.doi10.1002/jor.24479
dc.relation.ispartofjournalJournal of Orthopaedic Research
dc.relation.issue3
dc.year.issued2019


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