Synthesis of Aminoglycoside-2 '-O-Methyl Oligoribonucleotide Fusions
Tähtinen V; Virta P; Granqvist L; Kraszewski A
Synthesis of Aminoglycoside-2 '-O-Methyl Oligoribonucleotide Fusions
Tähtinen V
Virta P
Granqvist L
Kraszewski A
MDPI AG
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042717046
https://urn.fi/URN:NBN:fi-fe2021042717046
Tiivistelmä
Phosphoramidite building blocks of ribostamycin (3 and 4), that may be incorporated at any position of the oligonucleotide sequence, were synthesized. The building blocks, together with a previously described neomycin-modified solid support, were applied for the preparation of aminoglycoside-2'-O-methyl oligoribonucleotide fusions. The fusions were used to clamp a single strand DNA sequence (a purine-rich strand of c-Myc promoter 1) to form triple helical 2'-O-methyl RNA/DNA-hybrid constructs. The potential of the aminoglycoside moieties to stabilize the triple helical constructs were studied by UV-melting profile analysis.
Kokoelmat
- Rinnakkaistallenteet [19207]