Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes
Peurla Markus; Jokitalo Eija; Sahlgren Cecilia; Belevich Ilya; Prabhakar Neeraj; Heiligenstein Xavier; Chang Huan-Cheng; Rosenholm Jessica M
Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes
Peurla Markus
Jokitalo Eija
Sahlgren Cecilia
Belevich Ilya
Prabhakar Neeraj
Heiligenstein Xavier
Chang Huan-Cheng
Rosenholm Jessica M
MDPI
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042821242
https://urn.fi/URN:NBN:fi-fe2021042821242
Tiivistelmä
Three-dimensional correlative light and electron microscopy (3D CLEM) is attaining popularity as a potential technique to explore the functional aspects of a cell together with high-resolution ultrastructural details across the cell volume. To perform such a 3D CLEM experiment, there is an imperative requirement for multi-modal probes that are both fluorescent and electron-dense. These multi-modal probes will serve as landmarks in matching up the large full cell volume datasets acquired by different imaging modalities. Fluorescent nanodiamonds (FNDs) are a unique nanosized, fluorescent, and electron-dense material from the nanocarbon family. We hereby propose a novel and straightforward method for executing 3D CLEM using FNDs as multi-modal landmarks. We demonstrate that FND is biocompatible and is easily identified both in living cell fluorescence imaging and in serial block-face scanning electron microscopy (SB-EM). We illustrate the method by registering multi-modal datasets.
Kokoelmat
- Rinnakkaistallenteet [19207]