Inherited Metabolic Memory of High-Fat Diet Impairs Testicular Fatty Acid Content and Sperm Parameters
Batterham RL; Starcevic K; Jarak I; Rato L; Masek T; Alves MG; Crisostomo L; Oliveira PF; Videira RA; Raposo JF
Inherited Metabolic Memory of High-Fat Diet Impairs Testicular Fatty Acid Content and Sperm Parameters
Batterham RL
Starcevic K
Jarak I
Rato L
Masek T
Alves MG
Crisostomo L
Oliveira PF
Videira RA
Raposo JF
Lataukset:
WILEY
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2022091258668
https://urn.fi/URN:NBN:fi-fe2022091258668
Tiivistelmä
Scope Exposure to a high-fat diet (HFD) from early-life is associated with a testicular metabolic signature link to abnormal sperm parameters up to two generations after exposure in mice. Hereby, this study describes a testicular lipid signature associate with "inherited metabolic memory" of exposure to HFD, persisting up to two generations in mice. Methods and Results Diet-challenged mice (n = 36) are randomly fed after weaning with standard chow (CTRL); HFD for 200 days or transient HFD (HFDt) (60 days of HFD + 140 days of standard chow). Subsequent generations (36 mice per generation) are fed with chow diet. Mice are euthanized 200 days post-weaning. Glucose homeostasis, serum hormones, testicular bioenergetics, and antioxidant enzyme activity are evaluated. Testicular lipid-related metabolites and fatty acids are characterized by H-1-NMR and GC-MS. Sons of HFD display impaired choline metabolism, mitochondrial activity, and antioxidant defenses, while grandsons show a shift in testicular omega 3/omega 6 ratio towards a pro-inflammatory environment. Grandsons of HFDt raise 3-hydroxybutyrate levels with possible implications to testicular insulin resistance. Sperm counts decrease in grandsons of HFD-exposed mice, regardless of the duration of exposure. Conclusion HFD-induced "inherited metabolic memory" alters testicular fatty acid metabolism with consequences to sperm parameters up to two generations.
Kokoelmat
- Rinnakkaistallenteet [19207]