Effectiveness of porous silicon nanoparticle treatment at inhibiting the migration of a heterogeneous glioma cell population
Abdalla Youssef; Tong Wing Yin; Mäkilä Ermei; Luo Youssef; Voelcker Nicolas H.; Day Bryan W
Effectiveness of porous silicon nanoparticle treatment at inhibiting the migration of a heterogeneous glioma cell population
Abdalla Youssef
Tong Wing Yin
Mäkilä Ermei
Luo Youssef
Voelcker Nicolas H.
Day Bryan W
BMC
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021093048666
https://urn.fi/URN:NBN:fi-fe2021093048666
Tiivistelmä
Background: Approximately 80% of brain tumours are gliomas. Despite treatment, patient mortality remains high due to local metastasis and relapse. It has been shown that transferrin-functionalised porous silicon nanoparticles (Tf@pSiNPs) can inhibit the migration of U87 glioma cells. However, the underlying mechanisms and the effect of glioma cell heterogeneity, which is a hallmark of the disease, on the efficacy ofTf@pSiNPs remains to be addressed.
Results: Here, we observed that Tf@pSiNPs inhibited heterogeneous patient-derived glioma cells' (WK1) migration across small perforations (3 mu m) by approximately 30%. A phenotypical characterisation of the migrated subpopulations revealed that the majority of them were nestin and fibroblast growth factor receptor 1 positive, an indication of their cancer stem cell origin. The treatment did not inhibit cell migration across large perforations (8 mu m), nor cytoskeleton formation. This is in agreement with our previous observations that cellular-volume regulation is a mediator of Tf@pSiNPs'cell migration inhibition. Since aquaporin 9 (AQP9) is closely linked to cellular-volume regulation, and is highly expressed in glioma, the effect of AQP9 expression on WK1 migration was investigated. We showed that WK1 migration is correlated to the differential expression patterns of AQP9. However, AQP9-silencing did not affect WK1 cell migration across perforations, nor the efficacy of cell migration inhibition mediated by Tf@pSiNPs, suggesting that AQP9 is not a mediator of the inhibition.
Conclusion: This in vitro investigation highlights the unique therapeutic potentials ofTf@pSiNPs against glioma cell migration and indicates further optimisations that are required to maximise its therapeutic efficacies.
Kokoelmat
- Rinnakkaistallenteet [19207]