A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials
M. Aubert; J. Frisk; C.-E. Wilén; H. Abdelkader; M. Mennillo; ; J.E. Eriksson; J. Peltonen; H. Öblom; M. Toivakka; P. Ihalainen; E. Rosqvist; D. Soto Véliz; R. Österbacka; A.P. Venu; N. Sandler; R. Koppolu; E Niemelä
A low-cost paper-based platform for fast and reliable screening of cellular interactions with materials
M. Aubert
J. Frisk
C.-E. Wilén
H. Abdelkader
M. Mennillo
J.E. Eriksson
J. Peltonen
H. Öblom
M. Toivakka
P. Ihalainen
E. Rosqvist
D. Soto Véliz
R. Österbacka
A.P. Venu
N. Sandler
R. Koppolu
E Niemelä
ROYAL SOC CHEMISTRY
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042822273
https://urn.fi/URN:NBN:fi-fe2021042822273
Tiivistelmä
A paper-based platform was developed and tested for studies on basic cell culture, material biocompatibility, and activity of pharmaceuticals in order to provide a reliable, robust and low-cost cell study platform. It is based upon a paper or paperboard support, with a nanostructured Latex coating to provide an enhanced cell growth and sufficient barrier properties. Wetting is Limited to regions of interest using a flexographically printed hydrophobic polydimethylsiloxane Layer with circular non-print areas. The nanostructured coating can be substituted for another coating of interest, or the regions of interest functionalized with a material to be studied. The platform is fully up-scalable, being produced with roll-to-roll rod coating, flexographic and inkjet printing methods. Results show that the platform efficiency is comparable to multi-well plates in colorimetric assays in three separate studies: a cell culture study, a biocompatibility study, and a drug screening study. The color intensity is quantified by using a common office scanner or an imaging device and the data is analyzed by a custom computer software without the need for expensive screening or analysis equipment.
Kokoelmat
- Rinnakkaistallenteet [19207]