dc.contributor.author | Yu | |
dc.contributor.author | Weishuai | |
dc.contributor.author | Papageorgiou Anastassios C. | |
dc.contributor.author | Li Duochuan | |
dc.contributor.author | Mohsin Imran | |
dc.date.accessioned | 2022-10-28T13:22:03Z | |
dc.date.available | 2022-10-28T13:22:03Z | |
dc.identifier.uri | https://www.utupub.fi/handle/10024/164667 | |
dc.description.abstract | <p>Auxiliary activity 9 (AA9) lytic polysaccharide monooxygenases (LPMOs) are copperdependent<br>oxidoreductases that use O2 or H2O2 to perform oxidative cleavage of cellulose in the<br>presence of an electron donor. Combined with cellulases, they can assist in a more efficient cleavage<br>of cellulose. AA9 LPMOs have therefore attracted considerable attention in recent years for use in<br>biotechnological applications. Here, a native AA9 LPMO (nTaAA9A) from the thermophilic fungus<br>Thermoascus aurantiacus was purified and characterized. The enzyme was shown to be active and able<br>to cleave cellulose and xylan to produce C1- and C4-oxidized products. It was also found to retain<br>about 84.3, 63.7, and 35.3% of its activity after incubation for 30 min at 60, 70, and 80 C, respectively,<br>using quantitative activity determination. The structure was determined to 1.36 Å resolution and<br>compared with that of the recombinant enzyme expressed in Aspergillus oryzae. Structural differences<br>in the glycosylated Asn138 and in solvent-exposed loops were identified.<br></p> | |
dc.language.iso | en | |
dc.title | Purification and Structural Characterization of the Auxiliary Activity 9 Native Lytic Polysaccharide Monooxygenase from Thermoascus aurantiacus and Identification of Its C1- and C4-Oxidized Reaction Products | |
dc.identifier.url | https://doi.org/10.3390/catal12020139 | |
dc.identifier.urn | URN:NBN:fi-fe2022021619452 | |
dc.relation.volume | 12 | |
dc.contributor.organization | fi=biotiedekeskuksen yhteiset|en=Biotiedekeskuksen yhteiset| | |
dc.contributor.organization-code | 2609200 | |
dc.converis.publication-id | 68728318 | |
dc.converis.url | https://research.utu.fi/converis/portal/Publication/68728318 | |
dc.format.pagerange | 1 | |
dc.format.pagerange | 14 | |
dc.identifier.eissn | 2073-4344 | |
dc.identifier.jour-issn | 2073-4344 | |
dc.okm.affiliatedauthor | Papageorgiou, Anastassios | |
dc.okm.affiliatedauthor | Imran, Mohsin | |
dc.okm.discipline | 1182 Biokemia, solu- ja molekyylibiologia | fi_FI |
dc.okm.discipline | 1182 Biochemistry, cell and molecular biology | en_GB |
dc.okm.internationalcopublication | international co-publication | |
dc.okm.internationality | International publication | |
dc.okm.type | Journal article | |
dc.publisher.country | Sveitsi | fi_FI |
dc.publisher.country | Switzerland | en_GB |
dc.publisher.country-code | CH | |
dc.relation.doi | 10.3390/catal12020139 | |
dc.relation.ispartofjournal | Catalysts | |
dc.relation.issue | 2 | |
dc.year.issued | 2022 | |