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Purification and Structural Characterization of the Auxiliary Activity 9 Native Lytic Polysaccharide Monooxygenase from Thermoascus aurantiacus and Identification of Its C1- and C4-Oxidized Reaction Products

Yu; Weishuai; Papageorgiou Anastassios C.; Li Duochuan; Mohsin Imran

dc.contributor.authorYu
dc.contributor.authorWeishuai
dc.contributor.authorPapageorgiou Anastassios C.
dc.contributor.authorLi Duochuan
dc.contributor.authorMohsin Imran
dc.date.accessioned2022-10-28T13:22:03Z
dc.date.available2022-10-28T13:22:03Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/164667
dc.description.abstract<p>Auxiliary activity 9 (AA9) lytic polysaccharide monooxygenases (LPMOs) are copperdependent<br>oxidoreductases that use O2 or H2O2 to perform oxidative cleavage of cellulose in the<br>presence of an electron donor. Combined with cellulases, they can assist in a more efficient cleavage<br>of cellulose. AA9 LPMOs have therefore attracted considerable attention in recent years for use in<br>biotechnological applications. Here, a native AA9 LPMO (nTaAA9A) from the thermophilic fungus<br>Thermoascus aurantiacus was purified and characterized. The enzyme was shown to be active and able<br>to cleave cellulose and xylan to produce C1- and C4-oxidized products. It was also found to retain<br>about 84.3, 63.7, and 35.3% of its activity after incubation for 30 min at 60, 70, and 80 C, respectively,<br>using quantitative activity determination. The structure was determined to 1.36 Å resolution and<br>compared with that of the recombinant enzyme expressed in Aspergillus oryzae. Structural differences<br>in the glycosylated Asn138 and in solvent-exposed loops were identified.<br></p>
dc.language.isoen
dc.titlePurification and Structural Characterization of the Auxiliary Activity 9 Native Lytic Polysaccharide Monooxygenase from Thermoascus aurantiacus and Identification of Its C1- and C4-Oxidized Reaction Products
dc.identifier.urlhttps://doi.org/10.3390/catal12020139
dc.identifier.urnURN:NBN:fi-fe2022021619452
dc.relation.volume12
dc.contributor.organizationfi=biotiedekeskuksen yhteiset|en=Biotiedekeskuksen yhteiset|
dc.contributor.organization-code2609200
dc.converis.publication-id68728318
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/68728318
dc.format.pagerange1
dc.format.pagerange14
dc.identifier.eissn2073-4344
dc.identifier.jour-issn2073-4344
dc.okm.affiliatedauthorPapageorgiou, Anastassios
dc.okm.affiliatedauthorImran, Mohsin
dc.okm.discipline1182 Biokemia, solu- ja molekyylibiologiafi_FI
dc.okm.discipline1182 Biochemistry, cell and molecular biologyen_GB
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countrySveitsifi_FI
dc.publisher.countrySwitzerlanden_GB
dc.publisher.country-codeCH
dc.relation.doi10.3390/catal12020139
dc.relation.ispartofjournalCatalysts
dc.relation.issue2
dc.year.issued2022


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