Arginine Methyltransferase PRMT7 Deregulates Expression of RUNX1 Target Genes in T-Cell Acute Lymphoblastic Leukemia
Laukkanen Saara; Kauko Otto; Mäkinen Artturi; Lohi Olli; Nikkilä Atte; Seki Masafumi; Oksa Laura; Rokka Anne; Takita Junko; Haapaniemi Pekka; Heinäniemi Merja; Hyvärinen Noora
Arginine Methyltransferase PRMT7 Deregulates Expression of RUNX1 Target Genes in T-Cell Acute Lymphoblastic Leukemia
Laukkanen Saara
Kauko Otto
Mäkinen Artturi
Lohi Olli
Nikkilä Atte
Seki Masafumi
Oksa Laura
Rokka Anne
Takita Junko
Haapaniemi Pekka
Heinäniemi Merja
Hyvärinen Noora
MDPI
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2022081154354
https://urn.fi/URN:NBN:fi-fe2022081154354
Tiivistelmä
T-cell acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy with no well-established prognostic biomarkers. We examined the expression of protein arginine methyltransferases across hematological malignancies and discovered high levels of PRMT7 mRNA in T-ALL, particularly in the mature subtypes of T-ALL. The genetic deletion of PRMT7 by CRISPR-Cas9 reduced the colony formation of T-ALL cells and changed arginine monomethylation patterns in protein complexes associated with the RNA and DNA processing and the T-ALL pathogenesis. Among them was RUNX1, whose target gene expression was consequently deregulated. These results suggest that PRMT7 plays an active role in the pathogenesis of T-ALL.
Kokoelmat
- Rinnakkaistallenteet [19207]