Effect of water storage of E-glass fiber-reinforced composite on adhesion of Streptococcus mutans.
Söderling E.; Tanner J; Vallittu PK
https://urn.fi/URN:NBN:fi-fe2021042720430
Tiivistelmä
Abstract
This study
investigated the effect of water storage of fiber-reinforced composite
on the adhesion of Streptococcus mutans (S. mutans) and its ability to
stay adhered and multiply on the FRC. The materials (E-glass fibers and
denture base polymer) were stored in water for 14 or 30 days or left
dry. Water contact angles of the materials before and after water
storage were determined. Test specimens, with or without parotid saliva
or serum pellicle, were incubated in a suspension of S. mutans allowing
initial adhesion to occur. Bacterial adhesion and multiplication was
studied using scanning electron microscopy. Contact angles of both
materials were significantly reduced after water storage indicating an
increase in surface free energy. When studied without a surface
pellicle, water storage significantly increased adhesion of S. mutans to
both glass and polymer. Saliva coating of the materials resulted in
higher degree of adhesion to glass fibers in comparison with polymer and
after 14 days water storage glass bound over twice as much S. mutans
cells than the polymer matrix. Bacterial growth and biofilm formation
occurred equally on both materials. The results of this in vitro study
suggest that in order to avoid the possible increase in S. mutans
adhesion, the reinforcing glass fibers should be covered with the matrix
polymer of the composite.
Kokoelmat
- Rinnakkaistallenteet [19207]