Controlled Monofunctionalization of Molecular Spherical Nucleic Acids on a Buckminster Fullerene Core
Gulumkar Vijay; Korhonen Heidi; Korsoff Niko; Leimu Laura; Mikkola Satu; Moisio Olli; Nesati Victor; Poijärvi-Virta Päivi; Rahkila Jani; Roivainen Anne; Tähtinen Ville; Viitala Tapani; Virta Pasi; Vuorimaa-Laukkanen Elina; Yliperttula Marjo; Äärelä Antti
Controlled Monofunctionalization of Molecular Spherical Nucleic Acids on a Buckminster Fullerene Core
Gulumkar Vijay
Korhonen Heidi
Korsoff Niko
Leimu Laura
Mikkola Satu
Moisio Olli
Nesati Victor
Poijärvi-Virta Päivi
Rahkila Jani
Roivainen Anne
Tähtinen Ville
Viitala Tapani
Virta Pasi
Vuorimaa-Laukkanen Elina
Yliperttula Marjo
Äärelä Antti
AMER CHEMICAL SOC
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021093048683
https://urn.fi/URN:NBN:fi-fe2021093048683
Tiivistelmä
An azide-functionalized 12-armed Buckminster fullerene has been monosubstituted in organic media with a substoichiometric amount of cyclooctyne-modified oligonucleo-tides. Exposing the intermediate products then to the same reaction (i. e., strain-promoted alkyne-azide cycloaddition, SPAAC) with an excess of slightly different oligonucleotide constituents in an aqueous medium yields molecularly defined monofunctionalized spherical nucleic acids (SNAs). This procedure offers a controlled synthesis scheme in which one oligonucleotide arm can be functionalized with labels or other conjugate groups (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid, DOTA, and Alexa-488 demonstrated), whereas the rest of the 11 arms can be left unmodified or modified by other conjugate groups in order to decorate the SNAs' outer sphere. Extra attention has been paid to the homogeneity and authenticity of the C60-azide scaffold used for the assembly of full-armed SNAs.
Kokoelmat
- Rinnakkaistallenteet [19207]