Multiplexed High-Throughput Serological Assay for Human Enteroviruses
Lehtonen J; Knip M; Hytönen VP; Lempainen J; Veijola R; Laitinen OH; Hyöty H; Saarinen NVV
Multiplexed High-Throughput Serological Assay for Human Enteroviruses
Lehtonen J
Knip M
Hytönen VP
Lempainen J
Veijola R
Laitinen OH
Hyöty H
Saarinen NVV
MDPI
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042822729
https://urn.fi/URN:NBN:fi-fe2021042822729
Tiivistelmä
Immunological assays detecting antibodies against enteroviruses typically use a single enterovirus serotype as antigen. This limits the ability of such assays to detect antibodies against different enterovirus types and to detect possible type-specific variation in antibody responses. We set out to develop a multiplexed assay for simultaneous detection of antibodies against multiple enterovirus and rhinovirus types encompassing all human infecting species. Seven recombinant VP1 proteins from enteroviruses EV-A to EV-D and rhinoviruses RV-A to RV-C species were produced. Using Meso Scale Diagnostics U-PLEX platform we were able to study antibody reactions against these proteins as well as non-structural enterovirus proteins in a single well with 140 human serum samples. Adults had on average 33-fold stronger antibody responses to these antigens (p< 10(-11)) compared to children, but children had less cross-reactivity between different enterovirus types. The results suggest that this new high-throughput assay offers clear benefits in the evaluation of humoral enterovirus immunity in children, giving more exact information than assays that are based on a single enterovirus type as antigen.
Kokoelmat
- Rinnakkaistallenteet [19207]