Comparing serum protein levels can aid in differentiating HPV-negative and -positive oropharyngeal squamous cell carcinoma patients
Antti Mäkitie; Amy Dickinson; Stina Syrjänen; Jaana Hagström; Petri Mattila; Caj Haglund; Sakari Joenväärä; Reija Randén-Brady; Suvi Silén; Timo Carpén; Mayank Saraswat; Robert Silén; Tiialotta Tohmola
Comparing serum protein levels can aid in differentiating HPV-negative and -positive oropharyngeal squamous cell carcinoma patients
Antti Mäkitie
Amy Dickinson
Stina Syrjänen
Jaana Hagström
Petri Mattila
Caj Haglund
Sakari Joenväärä
Reija Randén-Brady
Suvi Silén
Timo Carpén
Mayank Saraswat
Robert Silén
Tiialotta Tohmola
PUBLIC LIBRARY SCIENCE
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042823188
https://urn.fi/URN:NBN:fi-fe2021042823188
Tiivistelmä
Background The surrogate immunohistochemical marker, p16(INK4a), is used in clinical practice to determine the high-risk human papillomavirus (HPV) status of oropharyngeal squamous cell carcinomas (OPSCC). With a specificity of 83%, this will misclassify some patients compared with direct HPV testing. Patients who are p16(INK4a)-positive but HPV DNA-negative, or RNA-negative, may be unsuitable for treatment de-escalation aimed at reducing treatment-related side effects. We aimed to identify cost-effective serum markers to improve decision making for patients at risk of misclassification by p16(INK4a)alone.
Methods Serum proteins from pre-treatment samples of 36 patients with OPSCC were identified and quantified using label-free mass spectrometry-based proteomics. HPV-status was determined using p16(INK4a)/HPV DNA and E6/E7 mRNA. Serum protein expressions were compared between groups of patients according to HPV status, using the unpaired t-test with a Benjamini-Hochberg correction. ROC curves (AUC) were calculated with SPSS (v25).
Results Of 174 serum proteins identified, complement component C7 (C7), apolipoprotein F (ApoF) and galectin-3-Binding Protein (LGALS3BP) significantly differed between HPV-positive and -negative tumors (AUC ranging from 0.84-0.87). ApoF levels were more than twice as high in the E6/E7 mRNA HPV-positive group than HPV-negative.
Conclusions Serum C7, ApoF and LGALS3BP levels discriminate between HPV-positive and HPV-negative OPSCC. Further studies are needed to validate these host immunity-related proteins as markers for HPV-associated OPSCC.
Kokoelmat
- Rinnakkaistallenteet [19207]