Tandem-Mass-Tag Based Proteomic Analysis Facilitates Analyzing Critical Factors of Porous Silicon Nanoparticles in Determining Their Biological Responses under Diseased Condition
Hongbo Zhang; Li Li; Elbadry Khalil; Wen Liu; Zehua Liu; Xianming Deng; Wenzhong Zhang; Xueyan Liu; Giulia Torrieri; Jingyi Su; Yaohui He; Yunzhan Li; Flavia Fontana; Yuanming Xiu; Wenhua Lian; Ermei Mäkilä; Jarno Salonen; Hélder A. Santos; Jouni Hirvonen
Tandem-Mass-Tag Based Proteomic Analysis Facilitates Analyzing Critical Factors of Porous Silicon Nanoparticles in Determining Their Biological Responses under Diseased Condition
Hongbo Zhang
Li Li
Elbadry Khalil
Wen Liu
Zehua Liu
Xianming Deng
Wenzhong Zhang
Xueyan Liu
Giulia Torrieri
Jingyi Su
Yaohui He
Yunzhan Li
Flavia Fontana
Yuanming Xiu
Wenhua Lian
Ermei Mäkilä
Jarno Salonen
Hélder A. Santos
Jouni Hirvonen
WILEY
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042823382
https://urn.fi/URN:NBN:fi-fe2021042823382
Tiivistelmä
The analysis of nanoparticles' biocompatibility and immunogenicity is mostly performed under a healthy condition. However, more clinically relevant evaluation conducted under pathological condition is less known. Here, the immunogenicity and bio-nano interactions of porous silicon nanoparticles (PSi NPs) are evaluated in an acute liver inflammation mice model. Interestingly, a new mechanism in which PSi NPs can remit the hepatocellular damage and inflammation activation in a surface dependent manner through protein corona formation, which perturbs the inflammation by capturing the pro-inflammatory signaling proteins that are inordinately excreted or exposed under pathological condition, is found. This signal sequestration further attenuates the nuclear factor kappa B pathway activation and cytokines production from macrophages. Hence, the study proposes a potential mechanism for elucidating the altered immunogenicity of nanomaterials under pathological conditions, which might further offer insights to establish harmonized standards for assessing the biosafety of biomaterials in a disease-specific or personalized manner.
Kokoelmat
- Rinnakkaistallenteet [19207]