Mass Spectrometry-based Serum Proteomics for Biomarker discovery and validation
David R. Goodlett; Petri Kouvonen; Riitta Lahesmaa; Robert Moulder; Santosh D. Bhosale
Mass Spectrometry-based Serum Proteomics for Biomarker discovery and validation
David R. Goodlett
Petri Kouvonen
Riitta Lahesmaa
Robert Moulder
Santosh D. Bhosale
Springer
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042716671
https://urn.fi/URN:NBN:fi-fe2021042716671
Tiivistelmä
Blood protein measurements are used frequently
in the clinic in the assessment of patient health. Nevertheless, there remains
the need for new biomarkers with better diagnostic specificities. With the
advent of improved technology for bioanalysis and the growth of biobanks including
collections from specific disease-risk cohorts, the plasma proteome has
remained a target of proteomics research towards the characterization of
disease related biomarkers. The following protocol presents a workflow for serum/plasma
proteomics including details of sample preparation both with and without immunoaffinity
depletion of the most abundant plasma proteins, and methodology for selected
reaction monitoring mass spectrometry validation.
Kokoelmat
- Rinnakkaistallenteet [19207]