Filopodome Mapping Identifies p130Cas as a Mechanosensitive Regulator of Filopodia Stability
Hellyeh Hamidi; Johanna Ivaska; Aki Stubb; Rafael Saup; Guillaume Jacquemet; Elena Kremneva; Mitro Miihkinen
Filopodome Mapping Identifies p130Cas as a Mechanosensitive Regulator of Filopodia Stability
Hellyeh Hamidi
Johanna Ivaska
Aki Stubb
Rafael Saup
Guillaume Jacquemet
Elena Kremneva
Mitro Miihkinen
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042824469
https://urn.fi/URN:NBN:fi-fe2021042824469
Tiivistelmä
in filopodia tips, predicts critical roles for PIs in regulating filopodia ultra-structure and function. Our mapping further reveals that filopodia adhesions consist of a unique set of proteins, the filopodome, that are distinct from classical nascent adhesions, focal adhesions, and fibrillar adhesions. Using live imaging, we observe that filopodia adhesions can give rise to nascent adhesions, which, in turn, form focal adhesions. We demonstrate that p130Cas (BCAR1) is recruited to filopodia tips via its C-terminal Cas family homology domain (CCHD) and acts as a mechanosensitive regulator of filopodia stability. Finally, we demonstrate that our map based on myosin-X-induced filopodia can be translated to endogenous filopodia and fascin- and IRSp53-mediated filopodia.
Kokoelmat
- Rinnakkaistallenteet [19207]