Quantifying RNA synthesis at rate-limiting steps of transcription using nascent RNA-sequencing data
Vihervaara Anniina; Chandrakumaran Sajitha; Sistonen Lea; Rabenius Adelina
Quantifying RNA synthesis at rate-limiting steps of transcription using nascent RNA-sequencing data
Vihervaara Anniina
Chandrakumaran Sajitha
Sistonen Lea
Rabenius Adelina
Cell Press
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2022081154749
https://urn.fi/URN:NBN:fi-fe2022081154749
Tiivistelmä
Nascent RNA-sequencing tracks transcription at nucleotide resolution. The genomic distribution of engaged transcription complexes, in turn, uncovers functional genomic regions. Here, we provide analytical steps to (1) identify transcribed regulatory elements de novo genome-wide, (2) quantify engaged transcription complexes at enhancers, promoter-proximal regions, divergent transcripts, gene bodies, and termination windows, and (3) measure distribution of transcription machineries and regulatory proteins across functional genomic regions. This protocol tracks engaged transcription complexes across functional genomic regions demonstrated in human K562 erythroleukemia cells.
Kokoelmat
- Rinnakkaistallenteet [19207]