Engineered neutrophil-derived exosome-like vesicles for targeted cancer therapy
Qian Hui; Zhang Hongbo; Pan Jianming; Mao Fei; Ji Cheng; Zhang Jiahui; Shi Hui; Xu Wenrong; Zhang Xu; Wang Dongqing; Santos Helder A.; Fang Xinjian
Engineered neutrophil-derived exosome-like vesicles for targeted cancer therapy
Qian Hui
Zhang Hongbo
Pan Jianming
Mao Fei
Ji Cheng
Zhang Jiahui
Shi Hui
Xu Wenrong
Zhang Xu
Wang Dongqing
Santos Helder A.
Fang Xinjian
AMER ASSOC ADVANCEMENT SCIENCE
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2022081154757
https://urn.fi/URN:NBN:fi-fe2022081154757
Tiivistelmä
Neutrophils are the most abundant innate immune cells in human circulation; however, their derived exosomes have been rarely studied for tumor treatment. Here, we reported that exosomes from neutrophils (N-Ex) induce tumor cell apoptosis by delivering cytotoxic proteins and activating caspase signaling pathway. In addition, we decorated N-Ex with superparamagnetic iron oxide nanoparticles ( SPIONs) to achieve higher tumor-targeting therapeutic effect. We further fabricated exosome-like nanovesicles from neutrophils (NNVs) at high yield. Compared with liposome-loaded doxorubicin (DOX) and natural NNVs, DOX-loaded NNVs show an improved inhibition of tumor cell proliferation. Moreover, DOX-loaded, SPION-decorated NNVs selectively accumulate at the tumor sites under an external magnetic field, effectively restraining tumor growth and extensively prolonging the survival rate in mice. Overall, a simple and effective method to engineer N-Ex and NNVs at clinical applicable scale was developed, which enables the efficient and safe drug delivery for targeted and combined tumor therapy.
Kokoelmat
- Rinnakkaistallenteet [19207]