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Regulation of cyclic electron flow by chloroplast NADPH-dependent thioredoxin system

Tikkanen M; Toivola J; Nikkanen L; Trotta A; Aro E; Diaz MG; Rintamäki E

dc.contributor.authorTikkanen M
dc.contributor.authorToivola J
dc.contributor.authorNikkanen L
dc.contributor.authorTrotta A
dc.contributor.authorAro E
dc.contributor.authorDiaz MG
dc.contributor.authorRintamäki E
dc.date.accessioned2022-10-28T14:04:25Z
dc.date.available2022-10-28T14:04:25Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/169193
dc.description.abstractLinear electron transport in the thylakoid membrane drives photosynthetic NADPH and ATP production, while cyclic electron flow (CEF) around photosystem I only promotes the translocation of protons from stroma to thylakoid lumen. The chloroplast NADH dehydrogenase-like complex (NDH) participates in one CEF route transferring electrons from ferredoxin back to the plastoquinone pool with concomitant proton pumping to the lumen. CEF has been proposed to balance the ratio of ATP/NADPH production and to control the redox poise particularly in fluctuating light conditions, but the mechanisms regulating the NDH complex remain unknown. We have investigated potential regulation of the CEF pathways by the chloroplast NADPH-thioredoxin reductase (NTRC) in vivo by using an Arabidopsis knockout line of NTRC as well as lines overexpressing NTRC. Here, we present biochemical and biophysical evidence showing that NTRC stimulates the activity of NDH-dependent CEF and is involved in the regulation of generation of proton motive force, thylakoid conductivity to protons, and redox balance between the thylakoid electron transfer chain and the stroma during changes in light conditions. Furthermore, protein?protein interaction assays suggest a putative thioredoxin-target site in close proximity to the ferredoxin-binding domain of NDH, thus providing a plausible mechanism for redox regulation of the NDH ferredoxin:plastoquinone oxidoreductase activity.
dc.language.isoen
dc.publisherJohn Wiley & Sons, Ltd
dc.titleRegulation of cyclic electron flow by chloroplast NADPH-dependent thioredoxin system
dc.identifier.urlhttps://doi.org/10.1002/pld3.93
dc.identifier.urnURN:NBN:fi-fe2021042720145
dc.relation.volume2
dc.contributor.organizationfi=PÄÄT Molekulaarinen kasvibiologia|en=PÄÄT Molecular Plant Biology|
dc.contributor.organization-code2606205
dc.converis.publication-id36595001
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/36595001
dc.identifier.eissn2475-4455
dc.identifier.jour-issn2475-4455
dc.okm.affiliatedauthorTikkanen, Mikko
dc.okm.affiliatedauthorAro, Eva-Mari
dc.okm.affiliatedauthorNikkanen, Lauri
dc.okm.affiliatedauthorTrotta, Andrea
dc.okm.affiliatedauthorRintamäki, Eevi
dc.okm.affiliatedauthorToivola, Jouni
dc.okm.affiliatedauthorGuinea Díaz, Manuel
dc.okm.discipline1183 Kasvibiologia, mikrobiologia, virologiafi_FI
dc.okm.discipline1183 Plant biology, microbiology, virologyen_GB
dc.okm.internationalcopublicationnot an international co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.relation.articlenumbere00093
dc.relation.doi10.1002/pld3.93
dc.relation.ispartofjournalPlant Direct
dc.relation.issue11
dc.year.issued2018


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