Lipidomic and Metabolomic Signature of Progression of Chronic Kidney Disease in Patients with Severe Obesity
Morales Enrique; Lanzon Borja; Duberg Daniel; Medina-Gomez Gema; Ruperez Fransico; Hyötyläinen Tuulia; de Pablos Ignacio; Martin-Taboada Marina; Castro-Alves Victor; Rodriguez Elias; Vila-Bedmar Rocio; Oresic Matej; Gomez Pilar
Lipidomic and Metabolomic Signature of Progression of Chronic Kidney Disease in Patients with Severe Obesity
Morales Enrique
Lanzon Borja
Duberg Daniel
Medina-Gomez Gema
Ruperez Fransico
Hyötyläinen Tuulia
de Pablos Ignacio
Martin-Taboada Marina
Castro-Alves Victor
Rodriguez Elias
Vila-Bedmar Rocio
Oresic Matej
Gomez Pilar
MDPI
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2022012710993
https://urn.fi/URN:NBN:fi-fe2022012710993
Tiivistelmä
Severe obesity is a major risk for chronic kidney disease (CKD). Early detection and careful monitoring of renal function are critical for the prevention of CKD during obesity, since biopsies are not performed in patients with CKD and diagnosis is dependent on the assessment of clinical parameters. To explore whether distinct lipid and metabolic signatures in obesity may signify early stages of pathogenesis toward CKD, liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-high resolution accurate mass-mass spectrometry (GC-HRAM-MS) analyses were performed in the serum and the urine of severely obese patients with and without CKD. Moreover, the impact of bariatric surgery (BS) in lipid and metabolic signature was also studied, through LC-MS and GC-HRAM-MS analyses in the serum and urine of patients with severe obesity and CKD before and after undergoing BS. Regarding patients with severe obesity and CKD compared to severely obese patients without CKD, serum lipidome analysis revealed significant differences in lipid signature. Furthermore, serum metabolomics profile revealed significant changes in specific amino acids, with isoleucine and tyrosine, increased in CKD patients compared with patients without CKD. LC-MS and GC-HRAM-MS analysis in serum of patients with severe obesity and CKD after BS showed downregulation of levels of triglycerides (TGs) and diglycerides (DGs) as well as a decrease in branched-chain amino acid (BCAA), lysine, threonine, proline, and serine. In addition, BS removed most of the correlations in CKD patients against biochemical parameters related to kidney dysfunction. Concerning urine analysis, hippuric acid, valine and glutamine were significantly decreased in urine from CKD patients after surgery. Interestingly, bariatric surgery did not restore all the lipid species, some of them decreased, hence drawing attention to them as potential targets for early diagnosis or therapeutic intervention. Results obtained in this study would justify the use of comprehensive mass spectrometry-based lipidomics to measure other lipids aside from conventional lipid profiles and to validate possible early markers of risk of CKD in patients with severe obesity.
Kokoelmat
- Rinnakkaistallenteet [19207]