Näytä suppeat kuvailutiedot

p62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response

Miura Nobuaki; Noshiro Daisuke; Mizushima Tsunehiro; Kageyama Shun; Lee Jin-A; Ohe Tomoyuki; Abe Manabu; Motohashi Hozumi; Miura Yoshiki; Okuda Shujiro; Sakimura Kenji; Noda Nobuo N; Kazuno Saiko; Tamura Naoki; Ueno Takashi; Eskelinen Eeva-Liisa Komatsu Masaaki; Ichimura Yoshinobu; Waguri Satoshi; Gudmundsson Sigurdur Runar; Sou Yu-Shin

dc.contributor.authorMiura Nobuaki
dc.contributor.authorNoshiro Daisuke
dc.contributor.authorMizushima Tsunehiro
dc.contributor.authorKageyama Shun
dc.contributor.authorLee Jin-A
dc.contributor.authorOhe Tomoyuki
dc.contributor.authorAbe Manabu
dc.contributor.authorMotohashi Hozumi
dc.contributor.authorMiura Yoshiki
dc.contributor.authorOkuda Shujiro
dc.contributor.authorSakimura Kenji
dc.contributor.authorNoda Nobuo N
dc.contributor.authorKazuno Saiko
dc.contributor.authorTamura Naoki
dc.contributor.authorUeno Takashi
dc.contributor.authorEskelinen Eeva-Liisa Komatsu Masaaki
dc.contributor.authorIchimura Yoshinobu
dc.contributor.authorWaguri Satoshi
dc.contributor.authorGudmundsson Sigurdur Runar
dc.contributor.authorSou Yu-Shin
dc.date.accessioned2022-10-28T14:23:25Z
dc.date.available2022-10-28T14:23:25Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/171078
dc.description.abstractAutophagy contributes to the selective degradation of liquid droplets, including the P-Granule, Ape1-complex and p62/SQSTM1-body, although the molecular mechanisms and physiological relevance of selective degradation remain unclear. In this report, we describe the properties of endogenous p62-bodies, the effect of autophagosome biogenesis on these bodies, and the in vivo significance of their turnover. p62-bodies are low-liquidity gels containing ubiquitin and core autophagy-related proteins. Multiple autophagosomes form on the p62-gels, and the interaction of autophagosome-localizing Atg8-proteins with p62 directs autophagosome formation toward the p62-gel. Keap1 also reversibly translocates to the p62-gels in a p62-binding dependent fashion to activate the transcription factor Nrf2. Mice deficient for Atg8-interaction-dependent selective autophagy show that impaired turnover of p62-gels leads to Nrf2 hyperactivation in vivo. These results indicate that p62-gels are not simple substrates for autophagy but serve as platforms for both autophagosome formation and anti-oxidative stress.
dc.language.isoen
dc.publisherSpringerNature
dc.titlep62/SQSTM1-droplet serves as a platform for autophagosome formation and anti-oxidative stress response
dc.identifier.urnURN:NBN:fi-fe2021042826334
dc.relation.volume12
dc.contributor.organizationfi=biolääketieteen laitos, yhteiset|en=Institute of Biomedicine|
dc.contributor.organization-code2607100
dc.converis.publication-id51245853
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/51245853
dc.identifier.eissn2041-1723
dc.identifier.jour-issn2041-1723
dc.okm.affiliatedauthorEskelinen, Eeva-Liisa
dc.okm.discipline1182 Biokemia, solu- ja molekyylibiologiafi_FI
dc.okm.discipline1182 Biochemistry, cell and molecular biologyen_GB
dc.okm.discipline3111 Biolääketieteetfi_FI
dc.okm.discipline3111 Biomedicineen_GB
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countryBritanniafi_FI
dc.publisher.countryUnited Kingdomen_GB
dc.publisher.country-codeGB
dc.relation.articlenumber16
dc.relation.doi10.1038/s41467-020-20185-1
dc.relation.ispartofjournalNature Communications
dc.relation.issue1
dc.year.issued2021


Aineistoon kuuluvat tiedostot

Thumbnail

Aineisto kuuluu seuraaviin kokoelmiin

Näytä suppeat kuvailutiedot