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Transcriptomic Analysis of Young and Old Erythrocytes of Fish

Nikinmaa MJ; Götting M

dc.contributor.authorNikinmaa MJ
dc.contributor.authorGötting M
dc.date.accessioned2022-10-28T14:26:25Z
dc.date.available2022-10-28T14:26:25Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/171371
dc.description.abstractUnderstanding gene expression changes over the lifespan of cells is of fundamental interest and gives important insights into processes related to maturation and aging. This study was undertaken to understand the global transcriptome changes associated with aging in fish erythrocytes. Fish erythrocytes retain their nuclei throughout their lifetime and they are transcriptionally and translationally active. However, they lose important functions during their lifespan in the circulation. We separated rainbow trout (Oncorhynchus mykiss) erythrocytes into young and old fractions using fixed angle-centrifugation and analyzed transcriptome changes using RNA sequencing (RNA-seq) technology and quantitative real-time PCR. We found 930 differentially expressed between young and old erythrocyte fractions; 889 of these showed higher transcript levels in young, while only 34 protein-coding genes had higher transcript levels in old erythrocytes. In particular genes involved in ion binding, signal transduction, membrane transport, and those encoding various enzyme classes are affected in old erythrocytes. The transcripts with higher levels in old erythrocytes were associated with seven different GO terms within biological processes and nine within molecular functions and cellular components, respectively. Our study furthermore found several highly abundant transcripts as well as a number of differentially expressed genes (DEGs) for which the protein products are currently not known revealing the gaps of knowledge in most non-mammalian vertebrates. Our data provide the first insight into changes involved in aging on the transcriptional level and thus opens new perspectives for the study of maturation processes in fish erythrocytes.
dc.language.isoen
dc.publisherFRONTIERS MEDIA SA
dc.titleTranscriptomic Analysis of Young and Old Erythrocytes of Fish
dc.identifier.urnURN:NBN:fi-fe2021042717961
dc.relation.volume8
dc.contributor.organizationfi=PÄÄT Eläinfysiologia|en=PÄÄT Eläinfysiologia|
dc.contributor.organization-code2606405
dc.converis.publication-id28538146
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/28538146
dc.format.pagerange11
dc.format.pagerange1
dc.identifier.jour-issn1664-042X
dc.okm.affiliatedauthorGötting, Miriam
dc.okm.affiliatedauthorNikinmaa, Mikko
dc.okm.discipline1184 Genetiikka, kehitysbiologia, fysiologiafi_FI
dc.okm.discipline1184 Genetics, developmental biology, physiologyen_GB
dc.okm.internationalcopublicationnot an international co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countrySveitsifi_FI
dc.publisher.countrySwitzerlanden_GB
dc.publisher.country-codeCH
dc.relation.articlenumberARTN 1046
dc.relation.doi10.3389/fphys.2017.01046
dc.relation.ispartofjournalFrontiers in Physiology
dc.year.issued2017


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