Role of fibroblast growth factor receptors (FGFR) and FGFR like-1 (FGFRL1) in mesenchymal stromal cell differentiation to osteoblasts and adipocytes
M. Jian; P. Härkönen; T. E. Kähkönen; H. K. Väänänen; K. K. Ivaska; K. G. Büki
https://urn.fi/URN:NBN:fi-fe2021042717432
Tiivistelmä
Fibroblast growth factors (FGF) and their receptors (FGFRs) regulate many developmental processes including differentiation of mesenchymal stromal cells (MSC). We developed two MSC lines capable of differentiating to osteoblasts and adipocytes and studied the role of FGFRs in this process. We identified FGFR2 and fibroblast growth factor receptor like-1 (FGFRL1) as possible actors in MSC differentiation with gene microarray and qRT-PCR. FGFR2 and FGFRL1 mRNA expression strongly increased during MSC differentiation to osteoblasts. FGF2 treatment, resulting in downregulation
of FGFR2, or silencing FGFR2 expression with siRNAs inhibited
osteoblast differentiation. During adipocyte differentiation expression
of FGFR1
and FGFRL1 increased and was down-regulated by FGF2. FGFR1 knockdown
inhibited adipocyte differentiation. Silencing FGFR2 and FGFR1 in MSCs
was associated with decreased FGFRL1 expression in osteoblasts and
adipocytes, respectively. Our results suggest that FGFR1 and FGFR2
regulate FGFRL1 expression. FGFRL1 may mediate or modulate FGFR
regulation of MSC differentiation together with FGFR2 in osteoblastic
and FGFR1 in adipocytic lineage.
Kokoelmat
- Rinnakkaistallenteet [19207]