Näytä suppeat kuvailutiedot

Microfluidic-Assisted Fabrication of Dual-Coated pH-Sensitive Mesoporous Silica Nanoparticles for Protein Delivery

Inam Wali; Küçüktürkmen Berrin; Zhang Hongbo; Prabhakar Neeraj; Howaili Fadak; Gouda Mariam; Rosenholm Jessica M.

dc.contributor.authorInam Wali
dc.contributor.authorKüçüktürkmen Berrin
dc.contributor.authorZhang Hongbo
dc.contributor.authorPrabhakar Neeraj
dc.contributor.authorHowaili Fadak
dc.contributor.authorGouda Mariam
dc.contributor.authorRosenholm Jessica M.
dc.date.accessioned2022-10-28T14:35:45Z
dc.date.available2022-10-28T14:35:45Z
dc.identifier.urihttps://www.utupub.fi/handle/10024/172266
dc.description.abstractMicrofluidics has become a popular method for constructing nanosystems in recent years, but it can also be used to coat other materials with polymeric layers. The polymeric coating may serve as a diffusion barrier against hydrophilic compounds, a responsive layer for controlled release, or a functional layer introduced to a nanocomposite for achieving the desired surface chemistry. In this study, mesoporous silica nanoparticles (MSNs) with enlarged pores were synthesized to achieve high protein loading combined with high protein retention within the MSN system with the aid of a microfluidic coating. Thus, MSNs were first coated with a cationic polyelectrolyte, poly (diallyldimethylammonium chloride) (PDDMA), and to potentially further control the protein release, a second coating of a pH-sensitive polymer (spermine-modified acetylated dextran, SpAcDEX) was deposited by a designed microfluidic device. The protective PDDMA layer was first formed under aqueous conditions, whereby the bioactivity of the protein could be maintained. The second coating polymer, SpAcDEX, was preferred to provide pH-sensitive protein release in the intracellular environment. The optimized formulation was effectively taken up by the cells along with the loaded protein cargo. This proof-of-concept study thus demonstrated that the use of microfluidic technologies for the design of protein delivery systems has great potential in terms of creating multicomponent systems and preserving protein stability.
dc.language.isoen
dc.publisherMDPI
dc.titleMicrofluidic-Assisted Fabrication of Dual-Coated pH-Sensitive Mesoporous Silica Nanoparticles for Protein Delivery
dc.identifier.urlhttps://www.mdpi.com/2079-6374/12/3/181
dc.identifier.urnURN:NBN:fi-fe2022081155065
dc.relation.volume12
dc.contributor.organizationfi=biotiedekeskuksen yhteiset|en=Biotiedekeskuksen yhteiset|
dc.contributor.organization-code2609200
dc.converis.publication-id175274836
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/175274836
dc.identifier.eissn2079-6374
dc.identifier.jour-issn2079-6374
dc.okm.affiliatedauthorZhang, Hongbo
dc.okm.discipline317 Pharmacyen_GB
dc.okm.discipline317 Farmasiafi_FI
dc.okm.discipline318 Medical biotechnologyen_GB
dc.okm.discipline318 Lääketieteen bioteknologiafi_FI
dc.okm.internationalcopublicationinternational co-publication
dc.okm.internationalityInternational publication
dc.okm.typeJournal article
dc.publisher.countrySwitzerlanden_GB
dc.publisher.countrySveitsifi_FI
dc.publisher.country-codeCH
dc.relation.articlenumber181
dc.relation.doi10.3390/bios12030181
dc.relation.ispartofjournalBiosensors
dc.relation.issue3
dc.year.issued2022


Aineistoon kuuluvat tiedostot

Thumbnail

Aineisto kuuluu seuraaviin kokoelmiin

Näytä suppeat kuvailutiedot