Covalently Palladated Oligonucleotides Through Oxidative Addition of Pd-0
Räisälä H.; Lönnberg T.
Covalently Palladated Oligonucleotides Through Oxidative Addition of Pd-0
Räisälä H.
Lönnberg T.
WILEY-V C H VERLAG GMBH
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042827481
https://urn.fi/URN:NBN:fi-fe2021042827481
Tiivistelmä
An 11-mer oligonucleotide incorporating a central (2-iodobenzoylamino)methyl residue has been synthesized and palladated by oxidative addition of Pd-2(dba)(3). UV melting profiles of the duplexes formed by the palladated oligonucleotide with its natural complements were biphasic and the higher melting temperatures (T-m) exhibited considerable hysteresis. CD spectra, in turn, resembled those of canonical B-type double helices. Two-step denaturation, with the "low-T-m"melting involving only canonical base pairs and the "high-T-m" melting involving also dissociation of a Pd-II-mediated base pair, appears the most likely explanation for the observed UV melting profiles. As the latter step in all cases takes place at a higher temperature than denaturation of natural duplexes of the same length, the putative Pd-II-mediated base pairs are stabilizing.
Kokoelmat
- Rinnakkaistallenteet [19207]