Hyppää sisältöön
    • Suomeksi
    • In English
  • Suomeksi
  • In English
  • Kirjaudu
Näytä aineisto 
  •   Etusivu
  • 3. UTUCris-artikkelit
  • Rinnakkaistallenteet
  • Näytä aineisto
  •   Etusivu
  • 3. UTUCris-artikkelit
  • Rinnakkaistallenteet
  • Näytä aineisto
JavaScript is disabled for your browser. Some features of this site may not work without it.

Impairment of peroxisomal APX and CAT activities increases protection of photosynthesis under oxidative stress

Fabricio E. L. Carvalho; Yugo Lima-Melo; Rachel H. V. Sousa; Marcia Margis-Pinheiro; Setsuko Komatsu; Joaquim A. G. Silveira; Vicente T. C. B. Alencar; Danilo M. Daloso

Impairment of peroxisomal APX and CAT activities increases protection of photosynthesis under oxidative stress

Fabricio E. L. Carvalho
Yugo Lima-Melo
Rachel H. V. Sousa
Marcia Margis-Pinheiro
Setsuko Komatsu
Joaquim A. G. Silveira
Vicente T. C. B. Alencar
Danilo M. Daloso
Katso/Avaa
Publisher's PDF (1.349Mb)
Lataukset: 

Oxford University Press
doi:10.1093/jxb/ery354
URI
https://doi.org/10.1093/jxb/ery354
Näytä kaikki kuvailutiedot
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2021042720521
Tiivistelmä

Retrograde signalling pathways that are triggered by changes in cellular redox homeostasis remain poorly understood.


Transformed rice plants that are deficient in peroxisomal ascorbate peroxidase APX4 (OsAPX4-RNAi) are


known to exhibit more effective protection of photosynthesis against oxidative stress than controls when catalase


(CAT) is inhibited, but the mechanisms involved have not been characterized. An in-depth physiological and proteomics


analysis was therefore performed on OsAPX4-RNAi CAT-inhibited rice plants. Loss of APX4 function led to an


increased abundance of several proteins that are involved in essential metabolic pathways, possibly as a result of


increased tissue H2O2 levels. Higher photosynthetic activities observed in the OsAPX4-RNAi plants under CAT inhibition


were accompanied by higher levels of Rubisco, higher maximum rates of Rubisco carboxylation, and increased


photochemical efficiencies, together with large increases in photosynthesis-related proteins. Large increases were


also observed in the levels of proteins involved in the ascorbate/glutathione cycle and in other antioxidant-related


pathways, and these changes may be important in the protection of photosynthesis in the OsAPX4-RNAi plants. Large


increases in the abundance of proteins localized in the nuclei and mitochondria were also observed, together with


increased levels of proteins involved in important cellular pathways, particularly protein translation. Taken together,


the results show that OsAPX4-RNAi plants exhibit significant metabolic reprogramming, which incorporates a more


effective antioxidant response to protect photosynthesis under conditions of impaired CAT activity.


Kokoelmat
  • Rinnakkaistallenteet [19207]

Turun yliopiston kirjasto | Turun yliopisto
julkaisut@utu.fi | Tietosuoja | Saavutettavuusseloste
 

 

Tämä kokoelma

JulkaisuajatTekijätNimekkeetAsiasanatTiedekuntaLaitosOppiaineYhteisöt ja kokoelmat

Omat tiedot

Kirjaudu sisäänRekisteröidy

Turun yliopiston kirjasto | Turun yliopisto
julkaisut@utu.fi | Tietosuoja | Saavutettavuusseloste