Volatile Compound Analysis of Sea Buckthorn Berries with GC-MS
Idogwu, Bartholomew (2025-06-02)
Volatile Compound Analysis of Sea Buckthorn Berries with GC-MS
(02.06.2025)
Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
suljettu
Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2025061670368
https://urn.fi/URN:NBN:fi-fe2025061670368
Tiivistelmä
Sea buckthorn berries (Hippophae rhamnoides) contain volatiles that attract sea buckthorn flies (Rhagoletis batava), pests that can cause significant damage to the berries.
This study aimed to analyse and compare the volatiles in sea buckthorn cultivars Terhi and Tytti at three stages of ripeness (unripe, semi-ripe, and ripe). A special emphasis was placed on the known volatiles that attract R. batava, and their variations across the ripening stages and locations. The berries were harvested from Paattinen, Vahto, and Perniö (Southwest Finland), and Kittilä and Karigasniemi (Northern Finland), where only ripe berries were collected. In Karigasniemi, only Tytti was harvested; in Vahto, for semi-ripe and unripe samples, only Terhi was harvested. Ripe berries were analysed in two forms: as homogenates and as whole berries. Semi-ripe and unripe berries were analysed only as whole berries. Volatile compounds were analysed using headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS).
A total of 23 volatiles previously identified as attractants to R. batava were detected in the samples. In homogenised ripe samples, Tytti had higher total volatile attractants in Paattinen Vahto, and Perniö, while Terhi had higher total volatile attractants in Kittilä. In whole berry ripe samples, Tytti had higher total volatile attractants in Paattinen and Perniö, while Terhi had higher total volatile attractants in Vahto and Kittilä. In semi-ripe samples, Tytti had higher total volatile attractants in Paattinen, while Terhi had higher total volatile attractants in Perniö. In unripe samples, Terhi had higher total volatile attractants in both Paattinen and Perniö. In terms of locations, in ripe samples, for Terhi, Kittilä had the highest total volatile attractants; and for Tytti, Perniö had the highest total volatile attractants. In semi-ripe and unripe samples, for both cultivars, Perniö consistently had the highest total volatile attractants. The volatile attractants profiles in SB berries varied significantly between cultivars and among harvest locations.
This study aimed to analyse and compare the volatiles in sea buckthorn cultivars Terhi and Tytti at three stages of ripeness (unripe, semi-ripe, and ripe). A special emphasis was placed on the known volatiles that attract R. batava, and their variations across the ripening stages and locations. The berries were harvested from Paattinen, Vahto, and Perniö (Southwest Finland), and Kittilä and Karigasniemi (Northern Finland), where only ripe berries were collected. In Karigasniemi, only Tytti was harvested; in Vahto, for semi-ripe and unripe samples, only Terhi was harvested. Ripe berries were analysed in two forms: as homogenates and as whole berries. Semi-ripe and unripe berries were analysed only as whole berries. Volatile compounds were analysed using headspace solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME-GC-MS).
A total of 23 volatiles previously identified as attractants to R. batava were detected in the samples. In homogenised ripe samples, Tytti had higher total volatile attractants in Paattinen Vahto, and Perniö, while Terhi had higher total volatile attractants in Kittilä. In whole berry ripe samples, Tytti had higher total volatile attractants in Paattinen and Perniö, while Terhi had higher total volatile attractants in Vahto and Kittilä. In semi-ripe samples, Tytti had higher total volatile attractants in Paattinen, while Terhi had higher total volatile attractants in Perniö. In unripe samples, Terhi had higher total volatile attractants in both Paattinen and Perniö. In terms of locations, in ripe samples, for Terhi, Kittilä had the highest total volatile attractants; and for Tytti, Perniö had the highest total volatile attractants. In semi-ripe and unripe samples, for both cultivars, Perniö consistently had the highest total volatile attractants. The volatile attractants profiles in SB berries varied significantly between cultivars and among harvest locations.