Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver

Mansikkala Tuomas; Ohigashi Takuji; Salo Miia H.; Hiltunen Anniina E.; Vuolteenaho Reetta; Sipilä Petra; Kuure Satu; Huttula Marko; Uusimaa Johanna; Hinttala Reetta; Miinalainen Ilkka; Kangas Salla; Patanen Minna
Scanning transmission soft X-ray spectromicroscopy of mouse kidney and liver

Mansikkala Tuomas
Ohigashi Takuji
Salo Miia H.
Hiltunen Anniina E.
Vuolteenaho Reetta
Sipilä Petra
Kuure Satu
Huttula Marko
Uusimaa Johanna
Hinttala Reetta
Miinalainen Ilkka
Kangas Salla
Patanen Minna
Katso/Avaa
Lataukset: 

Elsevier B.V.
doi:10.1016/j.elspec.2023.147368
URI
https://doi.org/10.1016/j.elspec.2023.147368
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Julkaisun pysyvä osoite on:
https://urn.fi/URN:NBN:fi-fe2025082792020
Tiivistelmä

Scanning transmission X-ray microscopy (STXM) in the soft X-ray range is well-suited to study ultrastructural features of mammalian soft tissues. Especially at the carbon 1s edge, the imaging contrast varies drastically across the edge due to rapid changes in the X-ray absorption cross-section of functional groups present in the tissue samples enabling label-free soft X-ray spectromicroscopic studies. We present STXM spectromicroscopic imaging of mouse kidney and liver tissues. We especially concentrate on ultrastructural abnormalities in genetically modified Slc17a5 mice. STXM is a promising technique to study storage diseases without chemical alteration due to staining agents, but sample preparation poses a challenge.

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