Dietary Escitalopram Reduces Movement Variability and Enhances Behavioral Predictability in Drosophila melanogaster

Abstract

<p>Behavioral individuality, often termed animal personality, reflects consistent patterns of behavioral variability across individuals. In fruit flies (Drosophila melanogaster), pharmacological and dietary manipulations affecting neuromodulatory systems have been shown to alter behavior, but their effects on behavioral predictability remain incompletely understood. Here, we investigated whether developmental dietary exposure to tryptophan (a serotonin precursor) or escitalopram (a selective serotonin reuptake inhibitor, SSRI) is associated with changes in lateralized turning behavior. Flies were reared from larval stages on supplemented media and tested in a Y-maze assay to assess movement predictability. Flies exposed to escitalopram displayed significantly reduced behavioral variability compared to controls, indicated by a lower median absolute deviation (MAD) of turning behavior, whereas tryptophan supplementation did not significantly affect variability. Because both compounds were tested at a single dietary dose and serotonergic activity was not directly measured, these findings should be interpreted as dose-specific behavioral effects rather than evidence of altered serotonergic tone or mechanism. Our results demonstrate that chronic developmental exposure to escitalopram is associated with increased behavioral predictability in fruit flies, highlighting the utility of high-throughput behavioral assays for detecting subtle pharmacologically induced changes in individual variability. Future studies incorporating dose–response designs and physiological validation will be required to establish underlying mechanisms.<br></p>