NMR-based metabolomics of interspecific hybrid strawberries
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Julkaisu on tekijänoikeussäännösten alainen. Teosta voi lukea ja tulostaa henkilökohtaista käyttöä varten. Käyttö kaupallisiin tarkoituksiin on kielletty.
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The breeding of garden strawberries (Fragaria × ananassa) has a great significance in the food industry due to its wide cultivation. Today, allelic diversity from two wild sister species, F. chiloensis and F. virginiana, is being reintroduced to the strawberry for the purpose of enhancing agronomic traits such as fruit quality, disease resistance, and resilience to environmental change. The change in climate conditions, specifically in the Nordic region, has necessitated the development of adaptive strawberry cultivars. Consequently, newly reconstructed garden strawberries were developed through the NORDFRUIT breeding program by LUKE (National Resources Institute Finland). The aim of this research was to characterize the polar metabolic profile of the new interspecific hybrid strawberries and to help find the most potential hybrids for further breeding into commercial cultivars.
Nuclear magnetic resonance (NMR) spectroscopy, known for its high reproducibility, quantitative and qualitative nature, is a robust tool widely applied in food metabolomics. NMR provides a comprehensive analysis of metabolites in organisms, with a detailed overview of the polar metabolic profile. In this study, polar metabolites (amino acids, organic acids, phenolics, and sugars) were extracted from freeze-dried strawberries and analyzed according to 1H NMR metabolomics experimental workflow.
1H NMR-based metabolomics workflow combined with multivariate statistical data analysis were used to investigate the polar metabolite composition of 163 individual hybrids from 13 progeny groups, cultivar ‘Aho’ and six commercial cultivars (‘Honeoye’, ‘Korona’, ‘Lumo’, ‘Polka’ and ‘Ria’). The results demonstrated the metabolite variation between and within progeny groups when compared to commercial cultivars. In this study, reconstructed hybrid strawberries were notably differentiated by their sugars (glucose, fructose and sucrose), organic acids (citrate and malate), and amino acids (alanine, glutamine and threonine) profiles from the commercial cultivars. Although there was variation between the commercial cultivars and the hybrids, individuals from hybrid progeny groups 2, 4 and 8 closely correlated to the commercial cultivars through their sugar and amino acid profiles, validating the potential of their commercialization. Through this study, the hybrid strawberries were identified with 38 new metabolites such as 2-heptanone, 2,3-butanediol and cis-aconitate which contributed most to the unique metabolic profile of hybrids and correlating to specific aroma properties and stress responses in strawberry metabolism.