Applications of the bacteriophage Mu in vitro transposition reaction and genome manipulation via electroporation of DNA transposition complexes

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dc.contributor.authorSaija Haapa-Paananen
dc.contributor.authorHarri Savilahti
dc.contributor.organizationfi=fysiologia ja genetiikka|en=Physiology and Genetics|
dc.contributor.organization-code1.2.246.10.2458963.20.70712835001
dc.converis.publication-id28692277
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/28692277
dc.date.accessioned2022-10-28T13:36:07Z
dc.date.available2022-10-28T13:36:07Z
dc.description.abstract<p>The capacity of transposable elements to insert into the genomes has been harnessed during the past decades to various in vitro and in vivo applications. This chapter describes in detail the general protocols and principles applicable for the Mu in vitro transposition reaction as well as the assembly of DNA transposition complexes that can be electroporated into bacterial cells to accomplish efficient gene delivery. These techniques with their modifications potentiate various gene and genome modification applications, which are discussed briefly here, and the reader is referred to the original publications for further details.<br></p>
dc.format.extent8
dc.format.pagerange279
dc.format.pagerange286
dc.identifier.eisbn978-1-4939-7343-9
dc.identifier.isbn978-1-4939-7341-5
dc.identifier.issn1064-3745
dc.identifier.olddbid183024
dc.identifier.oldhandle10024/166118
dc.identifier.urihttps://www.utupub.fi/handle/11111/58176
dc.identifier.urnURN:NBN:fi-fe2021042718090
dc.language.isoen
dc.okm.affiliatedauthorHaapa-Paananen, Saija
dc.okm.affiliatedauthorSavilahti, Harri
dc.okm.discipline1184 Genetics, developmental biology, physiologyen_GB
dc.okm.discipline1184 Genetiikka, kehitysbiologia, fysiologiafi_FI
dc.okm.internationalcopublicationnot an international co-publication
dc.okm.internationalityInternational publication
dc.okm.typeA3 Book
dc.publisherHumana Press
dc.publisher.countryUnited Statesen_GB
dc.publisher.countryYhdysvallat (USA)fi_FI
dc.publisher.country-codeUS
dc.publisher.isbn978-0-89603;978-1-58829;978-1-59259;978-1-59745;978-1-60327;978-1-60761;978-1-61737;978-1-61779;978-1-62703;978-1-934115;978-1-4939; 978-1-0716
dc.publisher.placeNew York
dc.relation.doi10.1007/978-1-4939-7343-9_20
dc.relation.ispartofseriesMethods in Molecular Biology
dc.relation.volume1681
dc.source.identifierhttps://www.utupub.fi/handle/10024/166118
dc.titleApplications of the bacteriophage Mu in vitro transposition reaction and genome manipulation via electroporation of DNA transposition complexes
dc.title.bookBacteriophages: Methods and Protocols, Volume 3
dc.year.issued2018

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