Protocol to study early lung developmental branching in mouse embryos using explant culture

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dc.contributor.authorTalvi, Salli
dc.contributor.authorJokinen, Johanna
dc.contributor.authorRantakari, Pia
dc.contributor.authorHeino, Jyrki
dc.contributor.organizationfi=InFLAMES Lippulaiva|en=InFLAMES Flagship|
dc.contributor.organizationfi=MediCity|en=MediCity|
dc.contributor.organizationfi=Turun biotiedekeskus|en=Turku Bioscience Centre|
dc.contributor.organizationfi=biokemia|en=Biochemistry|
dc.contributor.organizationfi=biolääketieteen laitos|en=Institute of Biomedicine|
dc.contributor.organization-code1.2.246.10.2458963.20.18586209670
dc.contributor.organization-code1.2.246.10.2458963.20.49728377729
dc.contributor.organization-code1.2.246.10.2458963.20.68445910604
dc.contributor.organization-code1.2.246.10.2458963.20.77952289591
dc.converis.publication-id457268614
dc.converis.urlhttps://research.utu.fi/converis/portal/Publication/457268614
dc.date.accessioned2025-08-27T23:28:56Z
dc.date.available2025-08-27T23:28:56Z
dc.description.abstract<p>Mouse lung branching morphogenesis creates epithelial tree structures required for respiration. Here, we present a protocol for studying mouse lung developmental branching using lung explant cultures. We describe steps for isolating lungs with a video at embryonic day 12.5 (E12.5) and culturing as an explant for 2 days. We also detail procedures for microscopic imaging on days 0–2 and analysis of peripheral lung buds. This technique has the potential to investigate lung development in various conditions. For complete details on the use and execution of this protocol, please refer to Talvi et al.1</p>
dc.identifier.eissn2666-1667
dc.identifier.jour-issn2666-1667
dc.identifier.olddbid204044
dc.identifier.oldhandle10024/187071
dc.identifier.urihttps://www.utupub.fi/handle/11111/52066
dc.identifier.urlhttps://doi.org/10.1016/j.xpro.2024.103198
dc.identifier.urnURN:NBN:fi-fe2025082790319
dc.language.isoen
dc.okm.affiliatedauthorTalvi, Salli
dc.okm.affiliatedauthorJokinen, Johanna
dc.okm.affiliatedauthorRantakari, Pia
dc.okm.affiliatedauthorHeino, Jyrki
dc.okm.affiliatedauthorDataimport, MediCity
dc.okm.discipline3111 Biomedicineen_GB
dc.okm.discipline3111 Biolääketieteetfi_FI
dc.okm.internationalcopublicationnot an international co-publication
dc.okm.internationalityInternational publication
dc.okm.typeA1 ScientificArticle
dc.publisherCell Press
dc.publisher.countryUnited Statesen_GB
dc.publisher.countryYhdysvallat (USA)fi_FI
dc.publisher.country-codeUS
dc.relation.articlenumber103198
dc.relation.doi10.1016/j.xpro.2024.103198
dc.relation.ispartofjournalSTAR protocols
dc.relation.issue3
dc.relation.volume5
dc.source.identifierhttps://www.utupub.fi/handle/10024/187071
dc.titleProtocol to study early lung developmental branching in mouse embryos using explant culture
dc.year.issued2024

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